PDF(Pubmed)
Abstract：
Bell pepper plants are sensitive to environmental changes and are significantly affected by abiotic factors such as UV-B radiation and cold, which reduce their yield and production. Various approaches, including omics data integration, have been employed to understand the mechanisms by which this crop copes with abiotic stress. This study aimed to find metabolic changes in bell pepper stems caused by UV-B radiation and cold by integrating omic data. Proteome and metabolome profiles were generated using liquid chromatography coupled with mass spectrometry, and data integration was performed in the plant metabolic pathway database. The combined stress of UV-B and cold induced the accumulation of proteins related to photosynthesis, mitochondrial electron transport, and a response to a stimulus. Further, the production of flavonoids and their glycosides, as well as affecting carbon metabolism, tetrapyrrole, and scopolamine pathways, were identified. We have made the first metabolic regulatory network map showing how bell pepper stems respond to cold and UV-B stress. We did this by looking at changes in proteins and metabolites that help with respiration, photosynthesis, and the buildup of photoprotective and antioxidant compounds.
摘要：
甜椒植株对环境变化敏感,受UV-B辐射和寒冷等非生物因素影响显著,这降低了产量和产量。各种方法，包括组学数据集成，已经被用来了解这种作物应对非生物胁迫的机制。这项研究旨在通过整合组学数据来发现UV-B辐射和寒冷引起的甜椒茎的代谢变化。使用液相色谱和质谱联用技术生成蛋白质组和代谢组谱,并在植物代谢途径数据库中进行数据整合。UV-B和寒冷的联合胁迫诱导了与光合作用相关的蛋白质的积累，线粒体电子传输，对刺激的反应。Further,黄酮类化合物及其糖苷的生产，以及影响碳代谢，四吡咯,和东pol碱途径，已确定。我们制作了第一张代谢调节网络图，显示了甜椒茎如何应对寒冷和UV-B胁迫。我们通过观察有助于呼吸的蛋白质和代谢物的变化来做到这一点，光合作用,以及光保护和抗氧化化合物的积累。
