PDF(Pubmed)
Abstract：
The WRKY gene family is ubiquitously distributed in plants, serving crucial functions in stress responses. Nevertheless, the structural organization and evolutionary dynamics of WRKY genes in cotton have not been fully elucidated. In this study, a total of 112, 119, 217, and 222 WRKY genes were identified in Gossypium arboreum, Gossypium raimondii, Gossypium hirsutum, and Gossypium barbadense, respectively. These 670 WRKY genes were categorized into seven distinct subgroups and unequally distributed across chromosomes. Examination of conserved motifs, domains, cis-acting elements, and gene architecture collectively highlighted the evolutionary conservation and divergence within the WRKY gene family in cotton. Analysis of synteny and collinearity further confirmed instances of expansion, duplication, and loss events among WRKY genes during cotton evolution. Furthermore, GhWRKY31 transgenic Arabidopsis exhibited heightened germination rates and longer root lengths under drought and salt stress. Silencing GhWRKY31 in cotton led to reduced levels of ABA, proline, POD, and SOD, along with downregulated expression of stress-responsive genes. Yeast one-hybrid and molecular docking assays confirmed the binding capacity of GhWRKY31 to the W box of GhABF1, GhDREB2, and GhRD29. The findings collectively offer a systematic and comprehensive insight into the evolutionary patterns of cotton WRKYs, proposing a suitable regulatory framework for developing cotton cultivars with enhanced resilience to drought and salinity stress.
摘要：
WRKY基因家族广泛分布于植物中,在应激反应中发挥关键作用。然而，棉花中WRKY基因的结构组织和进化动力学尚未完全阐明。在这项研究中,在植物园棉中总共鉴定出112、119、217和222个WRKY基因，灵蒙地棉,陆地棉,和巴巴多斯棉属，分别。这670个WRKY基因被分为七个不同的亚组，并且在染色体上分布不均。保守图案的检查，域,顺式作用元素，和基因结构共同强调了棉花中WRKY基因家族的进化保守性和分歧性。同质性和共线性的分析进一步证实了扩张的实例，重复，和棉花进化过程中WRKY基因之间的丢失事件。此外，GhWRKY31转基因拟南芥在干旱和盐胁迫下表现出更高的发芽率和更长的根长。在棉花中沉默GhWRKY31导致ABA水平降低，脯氨酸,POD,和SOD，随着应激反应基因表达下调。酵母单杂交和分子对接试验证实了GhWRKY31与GhABF1、GhDREB2和GhRD29的W盒的结合能力。这些发现共同提供了对棉花WRKYs进化模式的系统和全面的见解，提出了一个合适的调控框架，用于开发具有增强的抗旱性和盐分胁迫能力的棉花品种。
