PDF(Pubmed)
Abstract：
According to four field experiments, after the inoculation of Phaseolus vulgaris L. cultivar Ufimskaya with the commercial strain Bacillus subtilis 26D and the promising strain B. subtilis 10-4, it was found that inoculation with B. subtilis 10-4 improved seed productivity (SP) by 31-41% per plant, but only in dry years. In contrast, all 4 years of inoculation with B. subtilis 26D were ineffective or neutral. It was intended to determine the growing and biochemical characteristics of inoculated 7-day-old plants, which correlate with the field SP of bacterial preparations. The SP of inoculated plants (average of 4 years) correlated with root length (0.83), MDA content (-0.98), and catalase (CAT) activity in roots (-0.96) of week-old seedlings. High correlation coefficients between the H2O2 content in the roots and SP (0.89 and 0.77), as well as between the H2O2 content in shoots and SP (0.98 and 0.56), were observed only in two dry years, when the influence of bacteria was detected. These physiological indicators were identified as potential markers for predicting the effectiveness of the endophytic symbiosis between bean plants and B. subtilis strains. The findings may be used to develop effective microbial-based, eco-friendly technologies for bean production.
摘要：
根据四个现场实验，在用商业菌株枯草芽孢杆菌26D和有希望的菌株枯草芽孢杆菌10-4接种菜豆品种Ufimskaya后，发现接种枯草芽孢杆菌10-4可提高种子生产力（SP）31-41％每株植物，但只有在干燥的年份。相比之下,所有4年接种枯草芽孢杆菌26D均无效或中性。旨在确定接种7日龄植物的生长和生化特征，与细菌制剂的现场SP相关。接种植物的SP（4年平均）与根长（0.83）相关，MDA含量（-0.98），周龄幼苗根中的过氧化氢酶（CAT）活性（-0.96）。根中H2O2含量与SP之间的高相关系数（0.89和0.77），以及芽中的H2O2含量和SP（0.98和0.56）之间，只在干旱的两年里观察到，当检测到细菌的影响时。这些生理指标被鉴定为预测豆植物和枯草芽孢杆菌菌株之间的内生共生有效性的潜在标记。这些发现可用于开发有效的基于微生物的，豆类生产的环保技术。
