PDF(Pubmed)
Abstract：
Estimating the impact of pesticide residue bioaccessibility in fruits on dietary exposure is a complex task in human health risk assessment. This research investigated the bioaccessibility of ten commonly used and detected pesticides in bananas and mangoes, as well as the factors influencing it, using an in vitro model. The highest bioaccessibility was observed at pH levels of 2.5 and 6.5 in the gastric and intestinal stages, respectively. Bioaccessibility decreased significantly with increasing solid/liquid ratios for most pesticides. The consumption of protein and four dietary components (carbohydrates, protein, lipids, and dietary fiber) could significantly reduce pesticide bioaccessibility by 9.89-48.32% (p < 0.05). Bioaccessibility in oral and gastric stages among four populations followed the order of adults/the elderly > children > infants, due to decreasing concentrations of α-amylase and pepsin. Pesticides in bananas generally exhibited a higher bioaccessibility (18.65-82.97%) compared to that in mangoes (11.68-87.57%). Bioaccessibility showed a negative correlation with the Log P values of the target pesticide, while no clear relationship was found between bioaccessibility and initial pesticide concentrations. Incorporating bioaccessible pesticide concentrations into risk assessments could lower dietary risk estimates by 11.85-79.57%. Assessing human exposure to pesticides based on bioaccessibility would greatly improve the accuracy of the risk assessment.
摘要：
在人类健康风险评估中，估计水果中农药残留生物可及性对饮食暴露的影响是一项复杂的任务。这项研究调查了香蕉和芒果中十种常用和检测到的农药的生物可及性，以及影响它的因素，使用体外模型。在胃和肠阶段，在pH值为2.5和6.5时观察到最高的生物可及性，分别。大多数农药的生物可及性随着固液比的增加而显着降低。蛋白质和四种膳食成分(碳水化合物，蛋白质，脂质，和膳食纤维）可以显着降低农药的生物可得性9.89-48.32％（p<0.05）。四个人群在口腔和胃部阶段的生物可及性遵循成人/老人>儿童>婴儿的顺序，由于α-淀粉酶和胃蛋白酶的浓度降低。与芒果中的农药（11.68-87.57％）相比，香蕉中的农药通常表现出更高的生物可及性（18.65-82.97％）。生物可及性与目标农药的LogP值呈负相关,而生物可及性与初始农药浓度之间没有明确的关系。将生物可获得的农药浓度纳入风险评估可以将饮食风险估计值降低11.85-79.57％。根据生物可及性评估人类暴露于农药将大大提高风险评估的准确性。
