PDF(Pubmed)
Abstract：
Rambutan seeds are by-products generated from fruit-processing factories; the leftover seeds are buried in landfills, generating methane emissions. This work aimed to extract polysaccharides (POLS) from rambutan seeds by using subcritical water extraction (SWE). The effects of defatting pretreatment and operating parameters in SWE were investigated using a Box-Behnken design. The results show that defatting pretreatment significantly enriched the POLS yield, while it had no significant effect on the total sugar content. Using the desirability approach, the suitable feedstock for SWE was defatted rambutan seeds. The maximum desirability of 0.86 was found at a temperature range of 145-150 °C, an extraction time of 15 min, and a liquid-solid ratio of 10:1. The POLS yield and total sugar content were in the range of 52.33-55.63 g/100 g feedstock and 83.37-87.45 g/100 g POLS, respectively. The extracted POLS had an equivalent molecular weight of 413.70 kDa that could be used as an extender in plant-based products. In conclusion, the defatting pretreatment of rambutan seeds not only improved the POLS yield obtained via SWE but also generated additional lipids that could be utilized as an unconventional source of specialty fat.
摘要：
红毛丹种子是水果加工厂生产的副产品；剩余的种子被埋在垃圾填埋场，产生甲烷排放。这项工作旨在通过使用亚临界水提取（SWE）从红毛丹种子中提取多糖（POLS）。使用Box-Behnken设计研究了脱脂预处理和操作参数在SWE中的影响。结果表明,脱脂预处理显著提高了POLS产量,而对总糖含量没有显著影响。使用可取性方法，SWE的合适原料是脱脂红毛丹种子。在145-150°C的温度范围内发现最大可取性为0.86，提取时间为15分钟，液固比为10:1。POLS产量和总糖含量在52.33-55.63g/100g原料和83.37-87.45g/100gPOLS之间，分别。提取的POLS具有413.70kDa的等效分子量,其可用作基于植物的产品中的延伸剂。总之,红毛丹种子的脱脂预处理不仅提高了通过SWE获得的POLS产量，而且还产生了额外的脂质，可用作非常规的特种脂肪来源。
