PDF(Pubmed)
Abstract：
Interest in biodegradable implants has focused attention on the resorbable polymer polylactic acid. However, the risk of these materials promoting infection, especially in patients with existing pathologies, needs to be monitored. The enrichment of a bacterial adhesion medium with compounds that are associated with human pathologies can help in understanding how these components affect the development of infectious processes. Specifically, this work evaluates the influence of glucose and ketone bodies (in a diabetic context) on the adhesion dynamics of S. aureus to the biomaterial polylactic acid, employing different approaches and discussing the results based on the physical properties of the bacterial surface and its metabolic activity. The combination of ketoacidosis and hyperglycemia (GK2) appears to be the worst scenario: this system promotes a state of continuous bacterial colonization over time, suppressing the stationary phase of adhesion and strengthening the attachment of bacteria to the surface. In addition, these supplements cause a significant increase in the metabolic activity of the bacteria. Compared to non-enriched media, biofilm formation doubles under ketoacidosis conditions, while in the planktonic state, it is glucose that triggers metabolic activity, which is practically suppressed when only ketone components are present. Both information must be complementary to understand what can happen in a real system, where planktonic bacteria are the ones that initially colonize a surface, and, subsequently, these attached bacteria end up forming a biofilm. This information highlights the need for good monitoring of diabetic patients, especially if they use an implanted device made of PLA.
摘要：
对生物可降解植入物的兴趣已将注意力集中在可再吸收的聚合物聚乳酸上。然而,这些材料促进感染的风险，尤其是在现有病理的患者中，需要监控。细菌粘附培养基中与人类病理相关的化合物的富集可以帮助理解这些成分如何影响感染过程的发展。具体来说,这项工作评估了葡萄糖和酮体（在糖尿病背景下）对金黄色葡萄球菌对生物材料聚乳酸的粘附动力学的影响，采用不同的方法，并根据细菌表面的物理性质及其代谢活性讨论结果。酮症酸中毒和高血糖症（GK2）的组合似乎是最糟糕的情况：该系统随着时间的推移促进了细菌持续定植的状态，抑制粘附的固定相并加强细菌对表面的附着。此外,这些补充剂导致细菌的代谢活性显着增加。与非丰富媒体相比，在酮症酸中毒条件下生物膜形成加倍，当处于浮游状态时，是葡萄糖引发代谢活动，当仅存在酮组分时,这实际上被抑制。这两种信息都必须是互补的，才能理解真实系统中可能发生的事情，浮游细菌是最初在表面定殖的细菌，and,随后,这些附着的细菌最终形成了生物膜。这些信息突出了对糖尿病患者进行良好监测的必要性，特别是如果他们使用PLA制成的植入装置。
