PDF(Pubmed)
Abstract：
Two in vitro experiments were conducted to evaluate the effects of Centella asiatica extract (CAE) supplementation on the rumen\'s in vitro fermentation characteristics. In the first experiment, CAE with five concentrations (C: 0%; T1: 3.05%; T2: 6.1%; T3: 12.2%; and T4: 24.4% CAE in diet) was supplemented in the rumen fluid and incubated for 6, 24, and 48 h to determine the optimal dosage. The total gas and methane production increased in all incubation times, and the total volatile fatty acids increased at 6 and 48 h. Ammonia nitrogen, branched chain volatile fatty acids, acetate, and butyrate were increased by CAE supplementation. T1 was chosen as the optimal dosage based on the total volatile fatty acids, branched chain volatile fatty acids, and ammonia nitrogen production. The CAE with the identified optimal dosage (T1) was incubated to identify its effect on the rumen\'s in vitro degradability in the second experiment. The CAE supplementation did not influence the in vitro dry matter, crude protein, or neutral detergent fiber degradability. In conclusion, CAE has no CH4 abatement or digestion promotion effects. However, CAE could be utilized as a feed additive to increase the rumen\'s total volatile fatty acid production without an adverse effect on the in vitro dry matter, crude protein, or neutral detergent fiber degradability.
摘要：
通过两个体外试验,评价了添加积雪草提取物(CAE)对瘤胃体外发酵特性的影响。在第一个实验中,在瘤胃液中补充了五种浓度的CAE（C：0％；T1：3.05％；T2：6.1％；T3：12.2％；和T4：饮食中的24.4％CAE），并孵育6、24和48小时以确定最佳剂量。在所有培养时间内，总气体和甲烷产量都增加了，总挥发性脂肪酸在6和48小时增加。氨氮，支链挥发性脂肪酸，醋酸盐,通过补充CAE增加丁酸盐。根据总挥发性脂肪酸选择T1为最佳剂量，支链挥发性脂肪酸，和氨氮生产。在第二个实验中，将具有确定的最佳剂量（T1）的CAE孵育以确定其对瘤胃体外降解性的影响。补充CAE不会影响体外干物质，粗蛋白，或中性洗涤剂纤维降解性。总之,CAE没有CH4减排或促进消化的作用。然而,CAE可用作饲料添加剂，以增加瘤胃的总挥发性脂肪酸产量，而不会对体外干物质产生不利影响。粗蛋白，或中性洗涤剂纤维降解性。
