METHODS: We collected 498 fecal samples from Holstein calves on 10 different farms in four regions of Yunnan Province. Nested PCR and DNA sequencing were used to determine the infection, species and genotypes of Cryptosporidium spp. in these animals.
RESULTS: The overall occurrence of Cryptosporidium spp. in Holstein calves was 32.9% (164/498), and the prevalence in pre- and post-weaned calves was 33.5% (106/316) and 31.9% (58/182), respectively. Four Cryptosporidium species were identified in these animals, namely C. bovis (n = 119), C. parvum (n = 23), C. ryanae (n = 20) and C. andersoni (n = 2). Based on sequencing analysis of the 60 kDa glycoprotein gene of C. bovis, C. parvum and C. ryanae, six subtypes of C. bovis (XXVIe, XXVIb, XXVIf, XXVIa XXVIc and XXVId), two subtypes of C. parvum (IIdA19G1 and IIdA18G1) and four subtypes of C. ryanae (XXIf, XXId, XXIe and XXIg) were identified.
CONCLUSIONS: These results provide essential information to understand the infection rate, species diversity and genetic structure of Cryptosporidium spp. populations in Holstein pre-weaned and post-weaned calves in Yunnan Province. Further, the presence of IIdA18G1 and IIdA19G1 in C. parvum implies significant animal and public health concerns, which requires greater attention and more preventive measures.
方法:我们从云南省四个地区的10个不同农场的荷斯坦牛身上收集了498份粪便样本。巢式PCR和DNA测序用于确定感染,隐孢子虫的种类和基因型。在这些动物中。
结果:隐孢子虫的总体发生率。在荷斯坦小牛中,为32.9%(164/498),断奶前后小牛的患病率分别为33.5%(106/316)和31.9%(58/182),分别。在这些动物中发现了四种隐孢子虫,即C.bovis(n=119),C.parvum(n=23),C.ryanae(n=20)和C.andersoni(n=2)。基于牛梭菌60kDa糖蛋白基因的测序分析,C.parvum和C.ryanae,牛梭菌的六种亚型(XXVIe,XXVIB,XXVif,XXVIaXXVIc和XXVId),parvum的两个亚型(IIdA19G1和IIdA18G1)和Ryanae的四个亚型(XXIf,XXId,确定了XXIe和XXIg)。
结论:这些结果为了解感染率提供了必要的信息,隐孢子虫的物种多样性和遗传结构.云南省荷斯坦断奶前和断奶后小牛的种群。Further,在C.parvum中存在IIdA18G1和IIdA19G1意味着重大的动物和公共卫生问题,这需要更多的关注和更多的预防措施。