PDF(Pubmed)
Abstract：
The presence of potentially pathogenic bacteria on veterinary clinic surfaces may be problematic. In this study, we collected swab samples (Fisherbrand, double transport swabs with Stuart\'s liquid medium) and water samples from five veterinary rehabilitation clinics. Swabs and water samples were transported to a microbiology lab for processing. At the lab, swabs were used to inoculate Hardy\'s Cdiff Banana Broth (for Clostridium difficile [Cdiff]) and five different types of bacterial growth media, including Hardy CHROM MRSA agar (methicillin-resistant Staphylococcus aureus [MRSA] and S. pseudintermedius [SIM]), mannitol salt agar (S. aureus [SA]), eosin methylene blue agar (enterics [ENT]), Pseudomonas isolation agar (Pseudomonas spp. [PS]), and tryptic soy agar [TSA] (non-specific). The most prominent presumptive species cultured was Cdiff (on nearly 55% of swabs). Bacillus spp. and enteric bacteria were encountered on nearly 35% of swabs, with MRSA and SIM on just over 10% of swabs. The most contaminated sample site was harnesses/life jackets used with the underwater treadmill (33% of swabs). The underwater treadmill water had total bacterial counts from 1,600 to 2,800 cfu/mL. Of all presumptive bacterial species detected, SIM tends to be more pathogenic for dogs. Targeted cleaning/disinfecting in these clinics could help reduce risks for both animals and caregivers utilizing these clinics.
摘要：
兽医诊所表面上潜在致病细菌的存在可能是有问题的。在这项研究中,我们收集了拭子样本(Fisherbrand，带有斯图尔特液体介质的双运输拭子）和来自五个兽医康复诊所的水样。将拭子和水样运送到微生物实验室进行处理。在实验室,拭子用于接种Hardy'sCdiff香蕉肉汤（用于艰难梭菌[Cdiff]）和五种不同类型的细菌生长培养基，包括HardyCHROMMRSA琼脂（耐甲氧西林金黄色葡萄球菌[MRSA]和假中介链球菌[SIM]），甘露醇盐琼脂(S.金黄色葡萄球菌[SA])，曙红亚甲蓝琼脂（肠[ENT]），假单胞菌分离琼脂（假单胞菌属。[PS]),和胰蛋白酶大豆琼脂[TSA](非特异性)。培养的最突出的推定物种是Cdiff（在近55％的拭子上）。芽孢杆菌。在近35%的拭子上遇到了肠道细菌，MRSA和SIM在10%以上的拭子上。污染最严重的样本地点是水下跑步机使用的安全带/救生衣（占拭子的33％）。水下跑步机水的总细菌计数为1,600至2,800cfu/mL。在所有检测到的推定细菌物种中，SIM倾向于对狗更有致病性。在这些诊所中有针对性的清洁/消毒可以帮助降低使用这些诊所的动物和看护者的风险。
