PDF(Pubmed)
Abstract：
BACKGROUND: Originating from odontogenic tissue, Odontogenic cysts are pathological cavities lined with epithelial cells and surrounded by fibrous connective tissue. This study investigated expression of CITED1 protein in different types of odontogenic cysts.
METHODS: 40 keratocysts, 40 radicular cysts, and 40 dentigerous cysts were excised and processed for routine paraffin wax embedding protocol. Macroscopic and panoramic radiographies images were used for diagnosis. Demographical properties and dental parameters were recorded. Cystic tissues were stained with hematoxylin-eosin dye and CITED1 antibody. Semi-quantitative analysis was performed for immune staining. The protein-protein interaction network, hub gene detection and KEGG analysis were conducted using Cytoscape software.
RESULTS: Odontogenic keratocysts was imaged with 6-8 layered epithelial cells and fibrous cyst walls with inflammatory cells. Radicular cysts had stratified squamous epithelium with varying thickness, ciliated cells, and Rushton hyaline bodies. Dentigerous cysts presented hyperplastic non-keratinized epithelium, fibrous tissue, rete ridges, and inflammatory cells. CITED1 immunoexpression was highest in odontogenic keratocysts, followed by radicular cysts, and lowest in dentigerous cysts. Nuclear and cytoplasmic CITED1 expression was significantly elevated in odontogenic keratocysts compared to radicular and dentigerous cysts. The top five targets of CITED1 were identified, primarily showing enrichment in hormone and cancer related pathways.
CONCLUSIONS: Positive CITED1 expression in all three types of odontogenic cysts suggest a potential role for CITED1 in the pathogenesis of odontogenic cysts, particularly in keratocysts. Further investigations are needed to elucidate the exact mechanisms underlying the differential expression of CITED1 and its implications for the development and progression of odontogenic cysts.
METHODS: 40 keratocysts, 40 radicular cysts, and 40 dentigerous cysts were excised and processed for routine paraffin wax embedding protocol. Macroscopic and panoramic radiographies images were used for diagnosis. Demographical properties and dental parameters were recorded. Cystic tissues were stained with hematoxylin-eosin dye and CITED1 antibody. Semi-quantitative analysis was performed for immune staining. The protein-protein interaction network, hub gene detection and KEGG analysis were conducted using Cytoscape software.
RESULTS: Odontogenic keratocysts was imaged with 6-8 layered epithelial cells and fibrous cyst walls with inflammatory cells. Radicular cysts had stratified squamous epithelium with varying thickness, ciliated cells, and Rushton hyaline bodies. Dentigerous cysts presented hyperplastic non-keratinized epithelium, fibrous tissue, rete ridges, and inflammatory cells. CITED1 immunoexpression was highest in odontogenic keratocysts, followed by radicular cysts, and lowest in dentigerous cysts. Nuclear and cytoplasmic CITED1 expression was significantly elevated in odontogenic keratocysts compared to radicular and dentigerous cysts. The top five targets of CITED1 were identified, primarily showing enrichment in hormone and cancer related pathways.
CONCLUSIONS: Positive CITED1 expression in all three types of odontogenic cysts suggest a potential role for CITED1 in the pathogenesis of odontogenic cysts, particularly in keratocysts. Further investigations are needed to elucidate the exact mechanisms underlying the differential expression of CITED1 and its implications for the development and progression of odontogenic cysts.
摘要：
背景：起源于牙源性组织，牙源性囊肿是排列有上皮细胞并被纤维结缔组织包围的病理腔。本研究调查了CITED1蛋白在不同类型牙源性囊肿中的表达。
方法：40例角化囊肿，40根囊肿,和40个牙囊被切除和处理常规石蜡包埋方案。宏观和全景摄影图像用于诊断。记录人口统计学特征和牙齿参数。囊性组织用苏木精-伊红染料和CITED1抗体染色。对免疫染色进行半定量分析。蛋白质-蛋白质相互作用网络，使用Cytoscape软件进行hub基因检测和KEGG分析。
结果：牙源性角化囊肿用6-8层上皮细胞和带有炎性细胞的纤维囊肿壁成像。根性囊肿有不同厚度的复层鳞状上皮，纤毛细胞,还有Rushton玻璃的尸体.牙囊肿表现为增生性非角化上皮，纤维组织,rete脊,和炎症细胞。CITED1免疫表达在牙源性角化囊肿中最高，其次是神经根囊肿,和最低的牙质囊肿。与根性和牙质囊肿相比，牙源性角化囊肿中的核和细胞质CITED1表达显着升高。确定了CITED1的前五个目标，主要显示激素和癌症相关途径的富集。
结论：阳性CITED1在所有三种类型的牙源性囊肿中的表达表明，CITED1在牙源性囊肿的发病机理中具有潜在的作用，尤其是角化囊肿。需要进一步的研究来阐明CITED1差异表达的确切机制及其对牙源性囊肿的发生和发展的影响。
方法：40例角化囊肿，40根囊肿,和40个牙囊被切除和处理常规石蜡包埋方案。宏观和全景摄影图像用于诊断。记录人口统计学特征和牙齿参数。囊性组织用苏木精-伊红染料和CITED1抗体染色。对免疫染色进行半定量分析。蛋白质-蛋白质相互作用网络，使用Cytoscape软件进行hub基因检测和KEGG分析。
结果：牙源性角化囊肿用6-8层上皮细胞和带有炎性细胞的纤维囊肿壁成像。根性囊肿有不同厚度的复层鳞状上皮，纤毛细胞,还有Rushton玻璃的尸体.牙囊肿表现为增生性非角化上皮，纤维组织,rete脊,和炎症细胞。CITED1免疫表达在牙源性角化囊肿中最高，其次是神经根囊肿,和最低的牙质囊肿。与根性和牙质囊肿相比，牙源性角化囊肿中的核和细胞质CITED1表达显着升高。确定了CITED1的前五个目标，主要显示激素和癌症相关途径的富集。
结论：阳性CITED1在所有三种类型的牙源性囊肿中的表达表明，CITED1在牙源性囊肿的发病机理中具有潜在的作用，尤其是角化囊肿。需要进一步的研究来阐明CITED1差异表达的确切机制及其对牙源性囊肿的发生和发展的影响。
