PDF(Pubmed)
Abstract：
BACKGROUND: Malaria continues to wreak havoc on the well-being of the community. Resistant parasites are jeopardizing the treatment. This is a wake-up call for better medications. Folk plants are the key starting point for antimalarial drug discovery. After crushing and mixing the leaves of Coriandrum sativum with water, one cup of tea is drunk daily for a duration of three to five days as a remedy for malaria by local folks in Ethiopia. Additionally, in vitro experiments conducted on the plant leaf extract elsewhere have also demonstrated the plant\'s malaria parasite inhibitory effect. There has been no pharmacologic research to assert this endowment in animals, though. This experiment was aimed at evaluating the antimalarial efficacy of C. sativum in Plasmodium berghei infected mice.
METHODS: The plant\'s leaf was extracted using maceration with distilled water. The extract was examined for potential acute toxicity. An evaluation of secondary phytoconstituents was done. Standard antimalarial screening models (prophylactic, chemosuppressive, curative tests) were utilized to assess the antiplasmodial effect. In each test, thirty mice were organized into groups of five. To the three categories, the test substance was given at doses of 100, 200 and 400 mg/kg/day before or after the commencement of P. berghei infection. Positive and negative control mice were provided Chloroquine and distilled water, respectively. Rectal temperature, parasitemia, body weight, survival time and packed cell volume were ultimately assessed. Analysis of the data was performed using Statistical Package for Social Sciences.
RESULTS: No toxicity was manifested in mice. The extract demonstrated a significant inhibition of parasitemia (p < 0.05) in all the models. The inhibition of parasite load was highest with the upper dose in the suppressive test (82.74%) followed by the curative procedure (78.49%). Likewise, inhibition of hypothermia, weight loss hampering, improved survival and protection against hemolysis were elicited by the extract.
CONCLUSIONS: The results of our experimental study revealed that the aqueous crude leaf extract of C. sativum exhibits significant antimalarial efficacy in multiple in vivo models involving mice infected with P. berghei. Given this promising therapeutic attribute, in depth investigation on the plant is recommended.
METHODS: The plant\'s leaf was extracted using maceration with distilled water. The extract was examined for potential acute toxicity. An evaluation of secondary phytoconstituents was done. Standard antimalarial screening models (prophylactic, chemosuppressive, curative tests) were utilized to assess the antiplasmodial effect. In each test, thirty mice were organized into groups of five. To the three categories, the test substance was given at doses of 100, 200 and 400 mg/kg/day before or after the commencement of P. berghei infection. Positive and negative control mice were provided Chloroquine and distilled water, respectively. Rectal temperature, parasitemia, body weight, survival time and packed cell volume were ultimately assessed. Analysis of the data was performed using Statistical Package for Social Sciences.
RESULTS: No toxicity was manifested in mice. The extract demonstrated a significant inhibition of parasitemia (p < 0.05) in all the models. The inhibition of parasite load was highest with the upper dose in the suppressive test (82.74%) followed by the curative procedure (78.49%). Likewise, inhibition of hypothermia, weight loss hampering, improved survival and protection against hemolysis were elicited by the extract.
CONCLUSIONS: The results of our experimental study revealed that the aqueous crude leaf extract of C. sativum exhibits significant antimalarial efficacy in multiple in vivo models involving mice infected with P. berghei. Given this promising therapeutic attribute, in depth investigation on the plant is recommended.
摘要：
背景：疟疾继续对社区的福祉造成严重破坏。抗性寄生虫正在危及治疗。这是对更好的药物的警钟。民间植物是抗疟药物发现的关键起点。将Coriandrumsativum的叶子与水压碎并混合后，每天喝一杯茶，持续三到五天，作为埃塞俄比亚当地人治疗疟疾的一种方法。此外,在其他地方对植物叶提取物进行的体外实验也证明了该植物对疟疾寄生虫的抑制作用。没有药理学研究来证明这种动物的禀赋，不过。该实验旨在评估C.sativum在伯氏疟原虫感染的小鼠中的抗疟功效。
方法:使用蒸馏水浸渍提取植物的叶。检查提取物的潜在急性毒性。对次要植物成分进行了评价。标准抗疟药筛查模型(预防性，化学抑制,治愈性测试）用于评估抗疟原虫作用。在每次测试中，将30只小鼠组织成5只一组。对于这三个类别，试验物质的剂量为100、200和400mg/kg/天，在伯氏疟原虫感染开始之前或之后。向阳性和阴性对照小鼠提供氯喹和蒸馏水，分别。直肠温度,寄生虫血症,体重,最终评估存活时间和细胞体积.使用社会科学统计软件包进行数据分析。
结果:在小鼠中没有表现出毒性。提取物在所有模型中均显示出对寄生虫血症的显著抑制(p<0.05)。抑制试验中剂量较高（82.74％），其次是治疗程序（78.49％），对寄生虫负荷的抑制作用最高。同样，抑制低温，减肥妨碍，提取物提高了存活率和对溶血的保护作用。
结论：我们的实验研究结果表明，在涉及感染伯氏疟原虫的小鼠的多种体内模型中，苜蓿的水性粗叶提取物表现出显著的抗疟功效。鉴于这种有希望的治疗属性，建议对工厂进行深入调查。
方法:使用蒸馏水浸渍提取植物的叶。检查提取物的潜在急性毒性。对次要植物成分进行了评价。标准抗疟药筛查模型(预防性，化学抑制,治愈性测试）用于评估抗疟原虫作用。在每次测试中，将30只小鼠组织成5只一组。对于这三个类别，试验物质的剂量为100、200和400mg/kg/天，在伯氏疟原虫感染开始之前或之后。向阳性和阴性对照小鼠提供氯喹和蒸馏水，分别。直肠温度,寄生虫血症,体重,最终评估存活时间和细胞体积.使用社会科学统计软件包进行数据分析。
结果:在小鼠中没有表现出毒性。提取物在所有模型中均显示出对寄生虫血症的显著抑制(p<0.05)。抑制试验中剂量较高（82.74％），其次是治疗程序（78.49％），对寄生虫负荷的抑制作用最高。同样，抑制低温，减肥妨碍，提取物提高了存活率和对溶血的保护作用。
结论：我们的实验研究结果表明，在涉及感染伯氏疟原虫的小鼠的多种体内模型中，苜蓿的水性粗叶提取物表现出显著的抗疟功效。鉴于这种有希望的治疗属性，建议对工厂进行深入调查。
