Abstract：
PPy nanoparticles are widely employed as PTT agents, because of their exceptional near-infrared absorption properties. Nonetheless, the efficacy of PTT with PPy nanoparticles is hindered by a challenge, specifically, a lack of precise targeting. In this study, a PTT imaging agent was developed by combining NCQDs having bright green fluorescent properties with PPy nanoparticles along with the masking of folic acid to overcome the challenge of targeting. The synthesized PPy:NCQDs:FA nanocomposite, characterized by extraordinary photothermal property, was utilized for imaging of folate receptor positive (FA+) MCF-7 cancer cells through the emission of green fluorescence by NCQDs incorporated within the nanocomposite. Additionally, these nanoparticles demonstrated a good level of cell viability, exceeding 82 %, even at a concentration of 600 μg mL-1. Even the in vivo toxicity inspection of the nanocomposite exemplified no observed acute toxicity at experimental dosages of 1 and 3 mg per kg body weight. By subjecting MCF-7 cells, inoculated with 100 μg mL-1 of nanocomposite, to NIR laser irradiation for 5 min, a significant decline in cell viability was witnessed, establishing the photothermal therapeutic potency of the nanocomposite. The death of cancer cells induced by nanocomposite was verified through MTT assay, imaging of cells by NCQDs alone, with nanocomposite, and by live/dead cell Calcein AM/PI staining assay. Quantification of induced apoptosis post-laser treatment is conducted through staining with Annexin V-FITC/PI. These findings establish potential use of PPy:NCQDs:FA nanocomposite as versatile theranostic agents, capable of targeted bioimaging and treatment for cancer cells exhibiting folate receptors.
摘要：
PPy纳米颗粒被广泛用作PTT试剂，因为它们特殊的近红外吸收特性。尽管如此，PTT与PPy纳米颗粒的功效受到挑战的阻碍，具体来说,缺乏精确的目标。在这项研究中,PTT显像剂是通过将具有亮绿色荧光特性的NCQDs与PPy纳米粒子以及叶酸掩蔽相结合来开发的,以克服靶向的挑战。合成的PPy：NCQDs：FA纳米复合材料，具有非凡的光热特性，通过掺入纳米复合材料内的NCQD发射绿色荧光,用于叶酸受体阳性(FA+)MCF-7癌细胞的成像。此外,这些纳米粒子表现出良好的细胞活力，超过82%，即使浓度为600μgmL-1。即使是纳米复合材料的体内毒性检查，在每公斤体重1和3毫克的实验剂量下也没有观察到急性毒性。通过接受MCF-7细胞，接种100μgmL-1的纳米复合材料，对近红外激光照射5分钟，细胞活力显著下降，建立纳米复合材料的光热治疗效能。通过MTT法验证纳米复合材料诱导的癌细胞死亡,仅通过NCQDs对细胞进行成像，用纳米复合材料，和通过活/死细胞钙黄绿素AM/PI染色测定。激光治疗后诱导的细胞凋亡的定量通过用膜联蛋白V-FITC/PI染色进行。这些发现确立了PPy：NCQDs：FA纳米复合材料作为多功能治疗药物的潜在用途，能够靶向生物成像和治疗表现出叶酸受体的癌细胞。
