Abstract：
Commercial mammalian collagen-based membranes used for guided tissue regeneration (GTR) in periodontal defect repair still face significant challenges, including ethical concerns, cost-effectiveness, and limited capacity for periodontal bone regeneration. Herein, an enhanced biomimetic mineralized hydroxyapatite (HAp)-fish-scale collagen (FCOL)/chitosan (CS) nanofibrous membrane was developed. Specifically, eco-friendly and biocompatible collagen extracted from grass carp fish scales was co-electrospun with CS to produce a biomimetic extracellular matrix membrane. An enhanced biomimetic mineralized HAp coating provided abundant active calcium and phosphate sites, which promoted cell osteogenic differentiation, and showed greater in vivo absorption. In vitro experiments demonstrated that the HAp-FCOL/CS membranes exhibited desirable properties with no cytotoxicity, provided a mimetic microenvironment for stem cell recruitment, and induced periodontal ligament cell osteogenic differentiation. In rat periodontal defects, HAp-FCOL/CS membranes significantly promoted new periodontal bone formation and regeneration. The results of this study indicate that low-cost, eco-friendly, and biomimetic HAp-FCOL/CS membranes could be promising alternatives to GTR membranes for periodontal regeneration in the clinic.
摘要：
用于牙周缺损修复的引导组织再生（GTR）的商业哺乳动物胶原基膜仍然面临重大挑战，包括伦理问题，成本效益，牙周骨再生能力有限。在这里，开发了一种增强的仿生矿化羟基磷灰石（HAp）-鱼鳞胶原蛋白（FCOL）/壳聚糖（CS）纳米纤维膜。具体来说,从草鱼鱼鳞中提取的生态友好且生物相容的胶原蛋白与CS共静电纺丝以产生仿生的细胞外基质膜。增强的仿生矿化HAp涂层提供了丰富的活性钙和磷酸盐位点，促进细胞成骨分化，并显示更大的体内吸收。体外实验表明，HAp-FCOL/CS膜表现出理想的特性，没有细胞毒性，为干细胞募集提供了模拟的微环境，诱导牙周膜细胞成骨分化。在大鼠牙周缺损中，HAp-FCOL/CS膜显著促进牙周新骨的形成和再生。这项研究的结果表明，低成本，环保,仿生HAp-FCOL/CS膜可能是临床上用于牙周再生的GTR膜的有希望的替代品。
