Abstract：
The Baihe River, a tributary of the Yellow River located in the Ngawa Tibetan and Qiang Autonomous Prefecture in Northern Sichuan, is surrounded by natural resources suitable for animal development. However, the impact of livestock activities water microbiome in this area remains unexplored. This study collected water samples from areas with captive yaks and sheep (NS and YS) and compared them with water samples from Hongyuan Baihe River. Through amplicon sequencing, we investigated the impact of livestock activities on aquatic microorganisms. Diversity analysis, significance analysis, and microbial phenotype prediction indicated a significant decrease in microbial community diversity and function in the NS and YS groups. Pathogenic microorganisms such as Bacteroidales and Thelebolaceae and antibiotic-resistant bacteria genes such as Flavobacteriales and Burkholderiaceae were significantly higher in livestock breeding areas. Additionally, bacteria adapted to acidification, hypoxia, and eutrophication (e.g., Acidobacteria, Flavobacteriales, Deltaproteobacteria, Rhodobacterales) were more abundant in these areas. Our results demonstrate that livestock activities significantly alter the structure and function of microbial communities in surrounding water bodies, deteriorating water quality.
摘要：
白河,黄河支流，位于四川北部的纳加瓦藏族羌族自治州，被适合动物发展的自然资源所包围。然而,该地区牲畜活动水微生物组的影响仍未探索。本研究收集了圈养牦牛和绵羊（NS和YS）地区的水样，并将其与红原白河的水样进行了比较。通过扩增子测序，我们调查了牲畜活动对水生微生物的影响。多样性分析,意义分析,和微生物表型预测表明NS和YS组的微生物群落多样性和功能显着下降。在家畜养殖区，诸如拟杆菌和Thelebolaceae之类的病原微生物以及诸如黄杆菌和伯克氏菌科之类的抗生素抗性细菌基因明显较高。此外,适应酸化的细菌，缺氧,和富营养化(例如，酸杆菌,黄杆菌,Deltaproteobacteria,红霉素）在这些地区更为丰富。我们的结果表明，牲畜活动显着改变了周围水体中微生物群落的结构和功能，水质恶化。
