PDF(Pubmed)
Abstract：
Background. Previous research has shown that delivery mode can shape infant gut microbiome composition. However, mothers delivering by caesarean section routinely receive prophylactic antibiotics prior to delivery, resulting in antibiotic exposure to the infant via the placenta. Previously, only a small number of studies have examined the effect of delivery mode versus antibiotic exposure on the infant gut microbiome with mixed findings.Objective. We aimed to determine the effect of delivery mode compared to antibiotic use during labour and delivery on the infant and maternal gut microbiome at 6 weeks post-partum.Methodology. Twenty-five mother-infant dyads were selected from the longitudinal Queensland Family Cohort Study. The selected dyads comprised nine vaginally delivered infants without antibiotics, seven vaginally delivered infants exposed to antibiotics and nine infants born by caesarean section with routine maternal prophylactic antibiotics. Shotgun-metagenomic sequencing of DNA from stool samples collected at 6 weeks post-partum from mother and infant was used to assess microbiome composition.Results. Caesarean section infants exhibited decreases in Bacteroidetes (ANCOM-BC q<0.0001, MaAsLin 2 q=0.041), changes to several functional pathways and altered beta diversity (R 2=0.056, P=0.029), while minimal differences due to antibiotic exposure were detected. For mothers, caesarean delivery (P=0.0007) and antibiotic use (P=0.016) decreased the evenness of the gut microbiome at 6 weeks post-partum without changing beta diversity. Several taxa in the maternal microbiome were altered in association with antibiotic use, with few differentially abundant taxa associated with delivery mode.Conclusion. For infants, delivery mode appears to have a larger effect on gut microbiome composition at 6 weeks post-partum than intrapartum antibiotic exposure. For mothers, both delivery mode and intrapartum antibiotic use have a small effect on gut microbiome composition at 6 weeks post-partum.
摘要：
背景。先前的研究表明，分娩方式可以塑造婴儿肠道微生物组组成。然而,剖腹产分娩的母亲在分娩前常规接受预防性抗生素，导致婴儿通过胎盘接触抗生素。以前,只有少量的研究检查了分娩方式与抗生素暴露对婴儿肠道微生物组的影响,结果不一.Objective.我们旨在确定分娩模式与分娩期间抗生素使用相比对产后6周婴儿和母体肠道微生物组的影响。方法论。从纵向昆士兰家庭队列研究中选择了25个母婴二元组。选定的二元组包括9名阴道分娩的婴儿，没有抗生素，7例接受抗生素治疗的阴道分娩婴儿和9例接受常规母体预防性抗生素的剖腹产婴儿。使用产后6周从母亲和婴儿收集的粪便样品的DNA的Shotgun-宏基因组测序来评估微生物组组成。结果。剖腹产婴儿的拟杆菌减少（ANCOM-BCq<0.0001，MaAsLin2q=0.041），几种功能途径的改变和β多样性的改变(R2=0.056，P=0.029)，同时检测到由于抗生素暴露引起的最小差异。对于母亲们来说,剖宫产（P=0.0007）和抗生素使用（P=0.016）降低了产后6周肠道微生物组的均匀性，而不改变β多样性。母体微生物组中的几个分类单元与抗生素的使用有关，与递送模式相关的差异丰富类群很少。结论。对于婴儿，分娩模式在产后6周时对肠道微生物组组成的影响似乎大于产时抗生素暴露.对于母亲们来说,分娩方式和产时使用抗生素对产后6周时的肠道微生物组组成影响较小.
