PDF(Pubmed)
Abstract：
UNASSIGNED: To determine the relationship between visual sensitivities from white-on-white Goldmann size I to V stimuli and the underlying retinal ganglion cell (RGC) content in the non-human primate (NHP) experimental glaucoma model.
UNASSIGNED: Normative data were collected from 13 NHPs. Unilateral experimental glaucoma was induced in seven animals with the least variable fields who were monitored using optical coherence tomography and 30-2 full-threshold standard automated perimetry (SAP). At varying endpoints, animals were euthanized followed by perfusion fixation, and 1-mm retinal punches were obtained from 34 corresponding SAP locations. RGCs were immunolabeled with an antibody against an RNA-binding protein (RBPMS) marker and imaged using confocal microscopy. RGC counts from each location were then related to visual sensitivities for each stimulus size, after accounting for ocular magnification.
UNASSIGNED: At the endpoint, the circumpapillary retinal nerve fiber layer thickness for experimental glaucoma eyes ranged from 47 to 113 µm. RGC density in control eyes was greatest for the 4.24° sample (18,024 ± 6869 cells/mm2) and decreased with eccentricity. Visual sensitivity at each tested location followed that predicted by spatial summation, with the critical area increasing with eccentricity (slope = 0.0036, R2 = 0.44). The relationship between RGC counts and visual sensitivity was described using a two-line fit, where the intercept of the first segment and hinge points were dependent on eccentricity.
UNASSIGNED: In NHPs, SAP visual thresholds are related to the underlying RGCs. The resulting spatial summation based structure-function model can be used to estimate RGC content from any standard white-on-white stimulus size.
UNASSIGNED: Normative data were collected from 13 NHPs. Unilateral experimental glaucoma was induced in seven animals with the least variable fields who were monitored using optical coherence tomography and 30-2 full-threshold standard automated perimetry (SAP). At varying endpoints, animals were euthanized followed by perfusion fixation, and 1-mm retinal punches were obtained from 34 corresponding SAP locations. RGCs were immunolabeled with an antibody against an RNA-binding protein (RBPMS) marker and imaged using confocal microscopy. RGC counts from each location were then related to visual sensitivities for each stimulus size, after accounting for ocular magnification.
UNASSIGNED: At the endpoint, the circumpapillary retinal nerve fiber layer thickness for experimental glaucoma eyes ranged from 47 to 113 µm. RGC density in control eyes was greatest for the 4.24° sample (18,024 ± 6869 cells/mm2) and decreased with eccentricity. Visual sensitivity at each tested location followed that predicted by spatial summation, with the critical area increasing with eccentricity (slope = 0.0036, R2 = 0.44). The relationship between RGC counts and visual sensitivity was described using a two-line fit, where the intercept of the first segment and hinge points were dependent on eccentricity.
UNASSIGNED: In NHPs, SAP visual thresholds are related to the underlying RGCs. The resulting spatial summation based structure-function model can be used to estimate RGC content from any standard white-on-white stimulus size.
摘要：
■在非人灵长类动物（NHP）实验性青光眼模型中，确定白对白戈德曼I至V大小刺激的视觉敏感性与潜在的视网膜神经节细胞（RGC）含量之间的关系。
■从13个NHP收集规范数据。在使用光学相干断层扫描和30-2全阈值标准自动视野（SAP）监测的七只视野变化最小的动物中诱发了单侧实验性青光眼。在不同的端点，对动物实施安乐死，然后进行灌注固定，从34个相应的SAP位置获得1-mm视网膜穿孔。用针对RNA结合蛋白(RBPMS)标记物的抗体免疫标记RGC,并使用共聚焦显微镜成像。然后将每个位置的RGC计数与每个刺激大小的视觉敏感性相关，在考虑到眼睛放大后。
■在端点，实验性青光眼眼周乳头视网膜神经纤维层厚度为47~113µm.对于4.24°样品，对照眼中的RGC密度最大（18,024±6869个细胞/mm2），并且随着偏心率而降低。每个测试位置的视觉灵敏度，然后通过空间求和预测，临界面积随着偏心度的增加而增加(斜率=0.0036，R2=0.44)。使用两线拟合描述了RGC计数与视觉灵敏度之间的关系，其中第一段和铰接点的截距取决于偏心率。
■在NHPs中，SAP视觉阈值与基础RGC相关。所得到的基于空间求和的结构函数模型可用于从任何标准白上白上白上刺激大小估计RGC含量。
■从13个NHP收集规范数据。在使用光学相干断层扫描和30-2全阈值标准自动视野（SAP）监测的七只视野变化最小的动物中诱发了单侧实验性青光眼。在不同的端点，对动物实施安乐死，然后进行灌注固定，从34个相应的SAP位置获得1-mm视网膜穿孔。用针对RNA结合蛋白(RBPMS)标记物的抗体免疫标记RGC,并使用共聚焦显微镜成像。然后将每个位置的RGC计数与每个刺激大小的视觉敏感性相关，在考虑到眼睛放大后。
■在端点，实验性青光眼眼周乳头视网膜神经纤维层厚度为47~113µm.对于4.24°样品，对照眼中的RGC密度最大（18,024±6869个细胞/mm2），并且随着偏心率而降低。每个测试位置的视觉灵敏度，然后通过空间求和预测，临界面积随着偏心度的增加而增加(斜率=0.0036，R2=0.44)。使用两线拟合描述了RGC计数与视觉灵敏度之间的关系，其中第一段和铰接点的截距取决于偏心率。
■在NHPs中，SAP视觉阈值与基础RGC相关。所得到的基于空间求和的结构函数模型可用于从任何标准白上白上白上刺激大小估计RGC含量。
