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Abstract：
We developed an automated microregistration method that enables repeated in vivo skin microscopy imaging of the same tissue microlocation and specific cells over a long period of days and weeks with unprecedented precision. Applying this method in conjunction with an in vivo multimodality multiphoton microscope, the behavior of human skin cells such as cell proliferation, melanin upward migration, blood flow dynamics, and epidermal thickness adaptation can be recorded over time, facilitating quantitative cellular dynamics analysis. We demonstrated the usefulness of this method in a skin biology study by successfully monitoring skin cellular responses for a period of two weeks following an acute exposure to ultraviolet light.
摘要：
我们开发了一种自动化的显微配准方法，该方法可以在数天和数周的长时间内以前所未有的精度对相同的组织显微位置和特定细胞进行重复的体内皮肤显微镜成像。将此方法与体内多模态多光子显微镜结合使用，人类皮肤细胞的行为，如细胞增殖，黑色素向上迁移,血流动力学,随着时间的推移，可以记录表皮厚度的适应，促进定量细胞动力学分析。我们通过在急性暴露于紫外线后的两周内成功监测皮肤细胞反应，证明了该方法在皮肤生物学研究中的有用性。
