PDF(Pubmed)
Abstract：
Throughout embryonic development, the shaping of the functional and morphological characteristics of embryos is orchestrated by an intricate interaction between transcription factors and cis-regulatory elements. In this study, we conducted a comprehensive analysis of deuterostome cis-regulatory landscapes during gastrulation, focusing on four paradigmatic species: the echinoderm Strongylocentrotus purpuratus, the cephalochordate Branchiostoma lanceolatum, the urochordate Ciona intestinalis, and the vertebrate Danio rerio. Our approach involved comparative computational analysis of ATAC-seq datasets to explore the genome-wide blueprint of conserved transcription factor binding motifs underlying gastrulation. We identified a core set of conserved DNA binding motifs associated with 62 known transcription factors, indicating the remarkable conservation of the gastrulation regulatory landscape across deuterostomes. Our findings offer valuable insights into the evolutionary molecular dynamics of embryonic development, shedding light on conserved regulatory subprograms and providing a comprehensive perspective on the conservation and divergence of gene regulation underlying the gastrulation process.
摘要：
在整个胚胎发育过程中，胚胎功能和形态特征的形成是由转录因子和顺式调控元件之间复杂的相互作用所协调的。在这项研究中,我们在原肠胚形成过程中对子宫内膜顺式调节景观进行了全面分析，专注于四个典型物种：棘皮动物，头索状Branchiostomalaneolatum，泌尿肽Ciona肠，和脊椎动物Daniorerio.我们的方法涉及对ATAC-seq数据集的比较计算分析，以探索作为胃泌素基础的保守转录因子结合基序的全基因组蓝图。我们确定了一组与62个已知转录因子相关的保守DNA结合基序，表明整个子宫造口的原肠胚调节景观具有显着的保护作用。我们的发现为胚胎发育的进化分子动力学提供了有价值的见解，阐明了保守的调控子程序，并提供了有关原肠胚形成过程中基因调控的保守和分歧的全面观点。
