PDF(Pubmed)
Abstract：
Extracellular vesicles (EVs) are enclosed by a nanoscale phospholipid bilayer membrane and typically range in size from 30 to 200 nm. They contain a high concentration of specific proteins, nucleic acids, and lipids, reflecting but not identical to the composition of the parent cell. The inherent characteristics and variety of EVs give them extensive and unique advantages in the field of cancer identification and treatment. Recently, EVs have been recognized as potential tumor markers for the detection of cancer. Aptamers, which are molecules of single-stranded DNA or RNA, demonstrate remarkable specificity and affinity for their targets by adopting distinct tertiary structures. Aptamers offer various advantages over their protein counterparts, such as reduced immunogenicity, the ability for convenient large-scale synthesis, and straightforward chemical modification. In this review, we summarized EVs biogenesis, sample collection, isolation, storage and characterization, and finally provided a comprehensive survey of analysis techniques for EVs detection that are based on aptamers.
摘要：
细胞外囊泡(EV)被纳米级磷脂双层膜包围,并且通常在30至200nm的尺寸范围内。它们含有高浓度的特定蛋白质，核酸,和脂质，反映但不等同于亲本细胞的组成。电动汽车的固有特性和多样性使其在癌症识别和治疗领域具有广泛而独特的优势。最近,EV已被认为是检测癌症的潜在肿瘤标志物。适体,它们是单链DNA或RNA的分子，通过采用不同的三级结构，对它们的靶标表现出显著的特异性和亲和力。适体提供了多种优势比他们的蛋白质对应物，如降低免疫原性，方便大规模合成的能力，和直接的化学修饰。在这次审查中,我们总结了电动汽车的生物发生，样本采集,隔离,存储和表征，最后对基于适体的电动汽车检测分析技术进行了全面综述。
