UNASSIGNED: Experimental colonization of Ross 308 broilers with two different strains of C. jejuni was conducted, with groups fed either a basal diet or the same basal diet supplemented with 725 ppm algal extract from Saccharina latissima to provide 290 ppm laminarin. Fecal samples were collected for bacterial enumeration, and caecal samples were obtained before and after the C. jejuni challenge for the determination of microbiota development.
UNASSIGNED: No significant differences in fecal C. jejuni concentrations between the groups fed different diets or exposed to different C. jejuni strains were observed. This suggests that both strains colonized the birds equally well and that the laminarin rich algal extract did not have any inhibitory effect on C. jejuni colonization. Notably, 16S rRNA amplicon sequencing revealed detailed data on the caecal microbiota development, likely influenced by both bird age and C. jejuni colonization, which can be valuable for further development of broiler feed formulations aimed at promoting gut health.
■用两种不同菌株的空肠弯曲杆菌对罗斯308肉鸡进行了实验性定殖,各组饲喂基础饮食或相同的基础饮食,并补充了725ppm的来自拉氏糖藻的藻类提取物,以提供290ppm的海带多糖。收集粪便样本进行细菌计数,在空肠弯曲菌挑战之前和之后获得盲肠样品以确定微生物群发育。
■在饲喂不同饮食或暴露于不同空肠弯曲菌菌株的组之间没有观察到粪便空肠弯曲菌浓度的显著差异。这表明两种菌株同样良好地定殖鸟类,并且富含海带多糖的藻类提取物对空肠弯曲杆菌定殖没有任何抑制作用。值得注意的是,16SrRNA扩增子测序揭示了盲肠微生物群发育的详细数据,可能受鸟类年龄和空肠弯曲杆菌定植的影响,这对于进一步开发旨在促进肠道健康的肉鸡饲料配方是有价值的。