RESULTS: Using the threespine stickleback (Gasterosteus aculeatus) as a model, we confirmed that the genes for which nearby regions showed active marks, such as H3K4me1, H3K4me3, and high chromatin accessibility, were highly expressed. In contrast, the expression levels of genes for which nearby regions showed repressive marks, such as H3K27me3, were reduced, suggesting that our chromatin analysis protocols overall worked well. Genomic regions with peaks of histone modifications showed higher nucleotide diversity within and between populations. By comparing gene expression in the gills of the marine and stream ecotypes, we identified several insertions and deletions (indels) with transposable element fragments in the candidate cis-regulatory regions.
CONCLUSIONS: Thus, mapping and analyzing histone modifications can help identify cis-regulatory elements and accelerate the identification of causative mutations in the non-coding regions underlying naturally occurring phenotypic variations.
结果:使用三松棘鱼(Gasterosteusaculeatus)作为模型,我们证实了附近区域显示有活性标记的基因,如H3K4me1,H3K4me3和高染色质可及性,高度表达。相比之下,附近区域显示抑制标记的基因的表达水平,如H3K27me3,减少,表明我们的染色质分析方案总体运行良好。具有组蛋白修饰峰的基因组区域在种群内部和种群之间显示出更高的核苷酸多样性。通过比较海洋和河流生态型的g中的基因表达,我们在候选顺式调控区中鉴定了几个带有转座元件片段的插入和缺失(indel)。
结论:因此,定位和分析组蛋白修饰可以帮助识别顺式调控元件,并加速识别天然存在的表型变异的非编码区中的致病突变。