PDF(Pubmed)
Abstract：
β-glucans are polysaccharides found in the cell walls of various fungi, bacteria and cereals. β-glucan have been found to show various kinds of anti-inflammatory, antimicrobial, antidiabetic antioxidant and anticancerous activities. In the present study, we have isolated β-glucan from the baker\'s yeast Saccharomyces cerevisiae and white button mushroom Agaricus bisporus and tested their antioxidant potential and anticancerous activity against prostate cancer cell line PC3. Particles were characterized with zeta sizer and further with FTIR that confirmed that the isolated particles are β-glucan and alginate sealing made slow and sustained release of the Quercetin from the β-glucan particles. Morphological analysis of the hollow and Quercetin loaded β-glucan was performed with the SEM analysis and stability was analyzed with TGA and DSC analysis that showed the higher stability of the alginate sealed particles. Assessments of the antioxidant potential showed that Quercetin loaded particles were having higher antioxidant activity than hollow β-glucan particles. Cell viability of the PC3 cells was examined with MTT assay and it was found that Quercetin loaded alginate sealed Agaricus bisporus derived β-glucan particles were having lowest IC50. Further ROS generation was found to increase in a dose dependent manner. Apoptosis detection was carried out with Propidium iodide and AO/EtBr staining dye which showed significant death in the cells treated with higher concentration of the particles. Study showed that particles derived from both of the sources were having efficient anticancer activity and showing a dose dependent increase in cell death in PC3 cells upon treatment.
摘要：
β-葡聚糖是在各种真菌的细胞壁中发现的多糖，细菌和谷物。β-葡聚糖已被发现显示各种抗炎，抗菌，抗糖尿病抗氧化和抗癌活性。在本研究中,我们从面包师的酵母酿酒酵母和白色纽扣蘑菇双孢蘑菇中分离出β-葡聚糖，并测试了它们对前列腺癌细胞系PC3的抗氧化潜力和抗癌活性。用ζ分析仪和进一步用FTIR表征颗粒,其证实分离的颗粒是β-葡聚糖和藻酸盐密封,使得槲皮素从β-葡聚糖颗粒中缓慢和持续地释放。用SEM分析进行中空和装载槲皮素的β-葡聚糖的形态分析,并且用TGA和DSC分析进行稳定性分析,其显示藻酸盐密封颗粒的较高稳定性。抗氧化潜力的评估显示,负载槲皮素的颗粒具有比中空β-葡聚糖颗粒更高的抗氧化活性。用MTT测定法检查PC3细胞的细胞活力,发现负载槲皮素的藻酸盐密封的双孢蘑菇来源的β-葡聚糖颗粒具有最低的IC50。发现进一步的ROS产生以剂量依赖性方式增加。用碘化丙锭和AO/EtBr染色染料进行凋亡检测,其在用较高浓度的颗粒处理的细胞中显示出显著的死亡。研究表明,源自两种来源的颗粒具有有效的抗癌活性,并且在处理后显示PC3细胞中细胞死亡的剂量依赖性增加。
