PDF(Pubmed)
Abstract：
Red light (RL) can enhance egg production in poultry. CircRNAs play a crucial role by serving as transcriptional regulators. However, their role in influencing follicle development in White King pigeons remains unexplored. In this study, 54 paired White King pigeons were chosen and divided into RL and white light (WL) groups, each with 3 subgroups. The egg production of paired pigeons in each replicate was recorded for 45 d, and the characteristics of follicle development were monitored during the laying interval (LI). The granulosa cell layer from follicles of the second-largest follicle (F2) was collected, and high-throughput sequencing was performed to elucidate the molecular mechanism of follicle development in pigeons. The study confirmed that RL enhances egg production in pigeons. Additionally, under RL, the F2 follicle was selected, while under WL, small follicles were kept on the third day (LI3). A total of 5,510 circRNAs were identified across all samples, revealing differentially expressed circRNAs (DECs) in various comparisons: 627 in RF1 vs. WF1, 900 in RF2 vs. WF2, 606 in RF1 vs. RF2, and 937 in WF1 vs. WF2. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that host genes of DECs were enriched in pathways like steroid hormone biosynthesis, oocyte meiosis, GnRH signaling pathway, and apoptosis pathway. Moreover, circRNA_5497, circRNA_2016, and circRNA_3328 were common DECs across 4 groups, sharing miRNA binding sites with follicle selection-associated genes. In conclusion, our findings suggest that RL promotes egg production by stimulating follicle selection during LI, offering insights into the regulatory mechanisms of circRNAs in follicle selection under RL. This knowledge can help enhance the reproductive performance of pigeons.
摘要：
红光（RL）可以提高家禽的产蛋量。CircRNAs通过充当转录调节因子发挥关键作用。然而,它们在影响白王鸽卵泡发育中的作用仍未被探索。在这项研究中,选择了54只配对的白王鸽子，并分为RL和白光（WL）组，每个小组有3个小组。记录每个重复中配对鸽子的产蛋量45d，在产卵间隔（LI）期间监测卵泡发育特征。收集第二大卵泡（F2）卵泡的颗粒细胞层，并进行高通量测序以阐明鸽子卵泡发育的分子机制。研究证实，RL可以提高鸽子的产蛋量。此外,在RL下，选择了F2卵泡，在WL下，第3天保留小卵泡(LI3).在所有样品中总共鉴定了5,510个circRNAs，在各种比较中揭示差异表达的circRNAs（DEC）：RF1中的627与WF1，RF2中的900与WF2，RF1中的606与RF2和WF1中的937WF2。京都基因和基因组百科全书（KEGG）分析表明，DECs的宿主基因富集在类固醇激素生物合成等途径中，卵母细胞减数分裂,GnRH信号通路,和凋亡途径。此外，circRNA_5497、circRNA_2016和circRNA_3328是4组的常见DEC，与卵泡选择相关基因共享miRNA结合位点。总之,我们的发现表明RL通过在LI期间刺激卵泡选择来促进卵子产生，提供对RL条件下circRNAs在卵泡选择中的调控机制的见解。这些知识可以帮助提高鸽子的繁殖性能。
