Abstract：
UNASSIGNED: In order to achieve mutations with enhanced economic, productive, and nutritional characteristics in the two Egyptian cowpea varieties, Dokki 331 and Kaha 1, the application of gamma irradiation at different doses is employed. Additionally, this method aids in distinguishing between these mutations using simple sequence repeat (SSR) analysis.
UNASSIGNED: Two different cowpea cultivars were subjected to varying doses of gamma radiation ranging from 50 to 300 Gy. In order to analyze the effects of radiation, both unirradiated and irradiated seeds from both cultivars were planted using a randomized complete block design. This experiment was conducted over a span of six generations, namely M1, M2, M3, M4, M5, and M6, starting from April 2017 and continuing until 2022. Among the various radiation doses, the cultivar Kaha 1 produced promising traits when exposed to a dose of 150 Gy, while the cultivar Dokki 331 showed favorable traits when exposed to a dose of 300 Gy. These traits were further cultivated and studied until the M6 generation.
UNASSIGNED: Induced mutations in two Egyptian cowpea varieties, Kaha 1 and Dokki 331, are subjected to varying doses of gamma radiation (0, 50, 100, 150, 200, 250, and 300 Gy). Morphological and genetic variations were observed, with mutations being induced at doses of 150 Gy for Kaha 1 and 300 Gy for Dokki 331. The mutation in Kaha 1 (beam 1) resulted in dwarfism, altered leaf shape, early flowering, increased peduncles, pods, and pod seed numbers, ultimately leading to enhanced seed production and acreage productivity. In Dokki 331, the mutations primarily affected pod color, resulting in greenish-brown pods with mosaic seeds, segregating black and gray seeds from the mosaic ones. These mutations led to an increase in the nutritional value of the seeds, including higher nitrogen content, total free amino acids, crude protein, total carbohydrates, and total sugars. The genetic diversity of the seven cowpea mutations was assessed using 20 microsatellite markers. The analysis revealed a total of 60 alleles, with an average of three alleles per locus. The allele frequency ranged from 0.2857 to 1.0, with an average of 0.6036. Gene diversity varied from 0.0 to 0.8163, while the heterozygosity was mostly zero, except for one primer (VM 37) with an average of 0.0071. The polymorphic information content (PIC) ranged from 0.7913 to 0.0, with an average of 0.4323. The Marker Index value ranged from 0.36 to 0.0, with an average of 0.152. Overall, our findings demonstrate the successful induction of mutations in Egyptian cowpea varieties using gamma rays, resulting in improved yield characteristics and nutritional value.
UNASSIGNED: Radiation as a physical mutagen is highly regarded for its effectiveness, affordability, speed, and safety in inducing mutations. Utilizing gamma rays, we successfully derived a novel cowpea variety called beam 1 mutation, which has gained approval from the Egyptian Ministry of Agriculture.
UNASSIGNED: Two different cowpea cultivars were subjected to varying doses of gamma radiation ranging from 50 to 300 Gy. In order to analyze the effects of radiation, both unirradiated and irradiated seeds from both cultivars were planted using a randomized complete block design. This experiment was conducted over a span of six generations, namely M1, M2, M3, M4, M5, and M6, starting from April 2017 and continuing until 2022. Among the various radiation doses, the cultivar Kaha 1 produced promising traits when exposed to a dose of 150 Gy, while the cultivar Dokki 331 showed favorable traits when exposed to a dose of 300 Gy. These traits were further cultivated and studied until the M6 generation.
UNASSIGNED: Induced mutations in two Egyptian cowpea varieties, Kaha 1 and Dokki 331, are subjected to varying doses of gamma radiation (0, 50, 100, 150, 200, 250, and 300 Gy). Morphological and genetic variations were observed, with mutations being induced at doses of 150 Gy for Kaha 1 and 300 Gy for Dokki 331. The mutation in Kaha 1 (beam 1) resulted in dwarfism, altered leaf shape, early flowering, increased peduncles, pods, and pod seed numbers, ultimately leading to enhanced seed production and acreage productivity. In Dokki 331, the mutations primarily affected pod color, resulting in greenish-brown pods with mosaic seeds, segregating black and gray seeds from the mosaic ones. These mutations led to an increase in the nutritional value of the seeds, including higher nitrogen content, total free amino acids, crude protein, total carbohydrates, and total sugars. The genetic diversity of the seven cowpea mutations was assessed using 20 microsatellite markers. The analysis revealed a total of 60 alleles, with an average of three alleles per locus. The allele frequency ranged from 0.2857 to 1.0, with an average of 0.6036. Gene diversity varied from 0.0 to 0.8163, while the heterozygosity was mostly zero, except for one primer (VM 37) with an average of 0.0071. The polymorphic information content (PIC) ranged from 0.7913 to 0.0, with an average of 0.4323. The Marker Index value ranged from 0.36 to 0.0, with an average of 0.152. Overall, our findings demonstrate the successful induction of mutations in Egyptian cowpea varieties using gamma rays, resulting in improved yield characteristics and nutritional value.
UNASSIGNED: Radiation as a physical mutagen is highly regarded for its effectiveness, affordability, speed, and safety in inducing mutations. Utilizing gamma rays, we successfully derived a novel cowpea variety called beam 1 mutation, which has gained approval from the Egyptian Ministry of Agriculture.
摘要：
■为了实现经济增强的突变，生产,和两个埃及cow豆品种的营养特征，Dokki331和Kaha1，采用不同剂量的γ辐射。此外,该方法有助于使用简单序列重复(SSR)分析区分这些突变.
■对两个不同的of豆品种进行了50至300Gy的不同剂量的γ辐射。为了分析辐射的影响，使用随机完全区组设计种植来自两个品种的未辐照和辐照种子。这个实验进行了六代，即M1、M2、M3、M4、M5和M6，从2017年4月开始，一直持续到2022年。在各种辐射剂量中，当暴露于150Gy的剂量时，品种Kaha1产生了有希望的性状，而品种Dokki331在暴露于300Gy剂量时表现出良好的性状。这些性状被进一步培养和研究直到M6代。
■在两个埃及cow豆品种中诱导突变，Kaha1和Dokki331经受不同剂量的γ辐射(0、50、100、150、200、250和300Gy)。观察到形态和遗传变异，Kaha1的剂量为150Gy，Dokki331的剂量为300Gy。Kaha1（光束1）中的突变导致侏儒症，改变的叶子形状，早期开花，增加了花梗，豆荚,和豆荚种子编号，最终提高种子产量和种植面积。在Dokki331中，突变主要影响豆荚的颜色，产生带有马赛克种子的绿棕色豆荚，将黑色和灰色种子与马赛克种子分开。这些突变导致种子的营养价值增加，包括较高的氮含量，总游离氨基酸，粗蛋白，总碳水化合物，和总糖。使用20个微卫星标记评估了七个the豆突变的遗传多样性。分析显示总共有60个等位基因，每个基因座平均有三个等位基因。等位基因频率范围为0.2857至1.0，平均为0.6036。基因多样性从0.0到0.8163不等，而杂合度大多为零，除了一个引物（VM37），平均0.0071。多态信息含量(PIC)范围为0.7913~0.0,平均为0.4323。标记指数值范围为0.36至0.0,平均值为0.152。总的来说,我们的研究结果表明，利用伽马射线成功地诱导了埃及cow豆品种的突变，从而提高了产量特性和营养价值。
■辐射作为物理诱变剂因其有效性而备受推崇，负担能力，速度,和诱导突变的安全性。利用伽马射线,我们成功地衍生出了一种名为beam1突变的新型of豆品种，已获得埃及农业部的批准。
■对两个不同的of豆品种进行了50至300Gy的不同剂量的γ辐射。为了分析辐射的影响，使用随机完全区组设计种植来自两个品种的未辐照和辐照种子。这个实验进行了六代，即M1、M2、M3、M4、M5和M6，从2017年4月开始，一直持续到2022年。在各种辐射剂量中，当暴露于150Gy的剂量时，品种Kaha1产生了有希望的性状，而品种Dokki331在暴露于300Gy剂量时表现出良好的性状。这些性状被进一步培养和研究直到M6代。
■在两个埃及cow豆品种中诱导突变，Kaha1和Dokki331经受不同剂量的γ辐射(0、50、100、150、200、250和300Gy)。观察到形态和遗传变异，Kaha1的剂量为150Gy，Dokki331的剂量为300Gy。Kaha1（光束1）中的突变导致侏儒症，改变的叶子形状，早期开花，增加了花梗，豆荚,和豆荚种子编号，最终提高种子产量和种植面积。在Dokki331中，突变主要影响豆荚的颜色，产生带有马赛克种子的绿棕色豆荚，将黑色和灰色种子与马赛克种子分开。这些突变导致种子的营养价值增加，包括较高的氮含量，总游离氨基酸，粗蛋白，总碳水化合物，和总糖。使用20个微卫星标记评估了七个the豆突变的遗传多样性。分析显示总共有60个等位基因，每个基因座平均有三个等位基因。等位基因频率范围为0.2857至1.0，平均为0.6036。基因多样性从0.0到0.8163不等，而杂合度大多为零，除了一个引物（VM37），平均0.0071。多态信息含量(PIC)范围为0.7913~0.0,平均为0.4323。标记指数值范围为0.36至0.0,平均值为0.152。总的来说,我们的研究结果表明，利用伽马射线成功地诱导了埃及cow豆品种的突变，从而提高了产量特性和营养价值。
■辐射作为物理诱变剂因其有效性而备受推崇，负担能力，速度,和诱导突变的安全性。利用伽马射线,我们成功地衍生出了一种名为beam1突变的新型of豆品种，已获得埃及农业部的批准。
