Abstract：
BACKGROUND: Diagnosis of cutaneous leishmaniasis (CL) is difficult, and the correct use of histopathological criteria can be useful in clinical practice. The present study evaluates the association between histopathological findings and the results of polymerase chain reaction (PCR) in clinically suspected cases of CL.
METHODS: Skin samples were received in a laboratory from an endemic region of Brazil for over nine years. Associations were analyzed by means of the Chi square test with a 5% level of significance.
RESULTS: Of the 222 examined samples, 190 (85.6%) tested positive by PCR. All 25 cases identified by microscopic examination also tested positive by PCR. Except for the more intense inflammatory infiltrate, all other evaluated histological variables (ulceration, epidermal hyperplasia, hyperkeratosis, presence of granuloma, neutrophils, histiocytes, lymphocytes, plasmocytes, and necrosis) were not significantly associated with PCR positivity.
CONCLUSIONS: The intensity of the inflammatory infiltrate is a good indicator of the occurrence of CL. Histopathological aspects are useful to increase the predictive values of CL diagnoses, but PCR is still necessary to confirm or exclude the disease.
METHODS: Skin samples were received in a laboratory from an endemic region of Brazil for over nine years. Associations were analyzed by means of the Chi square test with a 5% level of significance.
RESULTS: Of the 222 examined samples, 190 (85.6%) tested positive by PCR. All 25 cases identified by microscopic examination also tested positive by PCR. Except for the more intense inflammatory infiltrate, all other evaluated histological variables (ulceration, epidermal hyperplasia, hyperkeratosis, presence of granuloma, neutrophils, histiocytes, lymphocytes, plasmocytes, and necrosis) were not significantly associated with PCR positivity.
CONCLUSIONS: The intensity of the inflammatory infiltrate is a good indicator of the occurrence of CL. Histopathological aspects are useful to increase the predictive values of CL diagnoses, but PCR is still necessary to confirm or exclude the disease.
摘要：
背景：皮肤利什曼病（CL）的诊断很困难，正确使用组织病理学标准可用于临床实践。本研究评估了临床可疑CL病例的组织病理学发现与聚合酶链反应（PCR）结果之间的关联。
方法：在实验室中从巴西的一个流行地区接收皮肤样本超过9年。通过卡方检验以5%的显著性水平分析关联。
结果：在222个检查样本中，190（85.6％）通过PCR检测为阳性。通过显微镜检查鉴定的所有25例病例也通过PCR检测为阳性。除了更强烈的炎症浸润，所有其他评估的组织学变量(溃疡，表皮增生,角化过度,肉芽肿的存在，中性粒细胞，组织细胞,淋巴细胞，浆细胞,和坏死）与PCR阳性没有显着相关。
结论：炎症浸润的强度是CL发生的良好指标。组织病理学方面有助于增加CL诊断的预测值，但PCR仍然需要确认或排除疾病。
方法：在实验室中从巴西的一个流行地区接收皮肤样本超过9年。通过卡方检验以5%的显著性水平分析关联。
结果：在222个检查样本中，190（85.6％）通过PCR检测为阳性。通过显微镜检查鉴定的所有25例病例也通过PCR检测为阳性。除了更强烈的炎症浸润，所有其他评估的组织学变量(溃疡，表皮增生,角化过度,肉芽肿的存在，中性粒细胞，组织细胞,淋巴细胞，浆细胞,和坏死）与PCR阳性没有显着相关。
结论：炎症浸润的强度是CL发生的良好指标。组织病理学方面有助于增加CL诊断的预测值，但PCR仍然需要确认或排除疾病。
