PDF(Pubmed)
Abstract：
BACKGROUND: The roadmap adopted by the World Health Organization (WHO) for eliminating neglected tropical diseases aims to eliminate schistosomiasis, as a public health concern, by 2030. While progress has been made towards reducing schistosomiasis morbidity control in several sub-Saharan African countries, there is still more that needs to be done. Proper surveillance using accurate diagnostics with acceptable sensitivity and specificity is essential for evaluating the success of all efforts against schistosomiasis. Microscopy, despite its low sensitivity, remains the gold standard approach for diagnosing the disease. Although many efforts have been made to develop new diagnostics based on circulating parasite proteins, genetic markers, schistosome egg morphology, and their paramagnetic properties, none has been robust enough to replace microscopy. This review highlights common diagnostic approaches for detecting schistosomiasis in field and clinical settings, major challenges, and provides new and novel opportunities and diagnosis pathways that will be critical in supporting elimination of schistosomiasis.
METHODS: We searched for relevant and reliable published literature from PubMed, Scopus, google scholar, and Web of science. The search strategies were primarily determined by subtopic, and hence the following words were used (schistosom*, diagnosis, Kato-Katz, antibody test, circulating antigen, POC-CCA, UCP-LF-CAA, molecular diagnostics, nucleic acid amplification test, microfluidics, lab-on a disk, lab-on chip, recombinase polymerase amplification (RPA), LAMP, portable sequencer, nanobody test, identical multi-repeat sequences, diagnostic TPPs, REASSURED, extraction free), and Boolean operators AND and/OR were used to refine the searching capacity. Due to the global public health nature of schistosomiasis, we also searched for reliable documents, reports, and research papers published by international health organizations, World Health Organization (WHO), and Center for Disease control and Elimination.
METHODS: We searched for relevant and reliable published literature from PubMed, Scopus, google scholar, and Web of science. The search strategies were primarily determined by subtopic, and hence the following words were used (schistosom*, diagnosis, Kato-Katz, antibody test, circulating antigen, POC-CCA, UCP-LF-CAA, molecular diagnostics, nucleic acid amplification test, microfluidics, lab-on a disk, lab-on chip, recombinase polymerase amplification (RPA), LAMP, portable sequencer, nanobody test, identical multi-repeat sequences, diagnostic TPPs, REASSURED, extraction free), and Boolean operators AND and/OR were used to refine the searching capacity. Due to the global public health nature of schistosomiasis, we also searched for reliable documents, reports, and research papers published by international health organizations, World Health Organization (WHO), and Center for Disease control and Elimination.
摘要：
背景：世界卫生组织（WHO）通过的消除被忽视的热带病的路线图旨在消除血吸虫病，作为公共卫生问题，到2030年。尽管撒哈拉以南非洲几个国家在减少血吸虫病发病率控制方面取得了进展，还有更多的事情需要做。使用具有可接受的灵敏度和特异性的准确诊断进行适当的监测对于评估所有针对血吸虫病的努力的成功至关重要。显微镜,尽管灵敏度低,仍然是诊断疾病的黄金标准方法。尽管已经做出了许多努力来开发基于循环寄生虫蛋白的新诊断方法，遗传标记,血吸虫卵形态，以及它们的顺磁性，没有一个足够强大，以取代显微镜。这篇综述重点介绍了在野外和临床环境中检测血吸虫病的常见诊断方法。重大挑战,并提供了新的和新颖的机会和诊断途径，这对于支持消除血吸虫病至关重要。
方法：我们搜索了PubMed的相关和可靠的已发表文献，Scopus,谷歌学者，和Web的科学。搜索策略主要由子主题决定，因此使用了以下词语(schistosom*，诊断,Kato-Katz,抗体测试,循环抗原，POC-CCA,UCP-LF-CAA,分子诊断，核酸扩增试验,微流体,实验室-在磁盘上，实验室芯片，重组酶聚合酶扩增（RPA），LAMP,便携式音序器,纳米抗体测试，相同的多重复序列，诊断TPP,已确认,无提取)，和布尔运算符AND和/OR用于优化搜索容量。由于血吸虫病的全球公共卫生性质，我们还搜索了可靠的文件，reports,和国际卫生组织发表的研究论文，世界卫生组织(世卫组织)和疾病控制和消除中心。
方法：我们搜索了PubMed的相关和可靠的已发表文献，Scopus,谷歌学者，和Web的科学。搜索策略主要由子主题决定，因此使用了以下词语(schistosom*，诊断,Kato-Katz,抗体测试,循环抗原，POC-CCA,UCP-LF-CAA,分子诊断，核酸扩增试验,微流体,实验室-在磁盘上，实验室芯片，重组酶聚合酶扩增（RPA），LAMP,便携式音序器,纳米抗体测试，相同的多重复序列，诊断TPP,已确认,无提取)，和布尔运算符AND和/OR用于优化搜索容量。由于血吸虫病的全球公共卫生性质，我们还搜索了可靠的文件，reports,和国际卫生组织发表的研究论文，世界卫生组织(世卫组织)和疾病控制和消除中心。
