Abstract：
Myxoma virus (MYXV) is a double-stranded DNA-containing virus of the family Poxviridae, genus Leporipoxvirus. MYXV is an important model virus for evolutionary and immunological research and a promising oncolytic. In this study, we sequenced and analyzed two complete genomes of MYXV virus vaccine strains B-82 and Rabbivac-B, which are widely used for vaccine production in Russia. Here, we first show that MYXV vaccine strains B-82 and Rabbivac-B share a common origin with the American recombinant MYXV MAV vaccine strain. In addition, our data suggest that the MYXV B-82 and Rabbivac-B strains contain a number of genes at the 5\' and 3\' ends that are identical to the virulent MYXV Lausanne strain. Several unique genetic signatures were identified in the M013L, M017L, M023, and M121R genes, helping to achieve high genetic resolution between vaccine strains. Overall, these findings highlight the evolutionary flexibility of certain genes in the MYXV genome and provide insights into the molecular epidemiology of the virus and subsequent vaccine development.
摘要：
粘液瘤病毒（MYXV）是Poxviridae家族的含双链DNA的病毒，Leporipoxvirus属。MYXV是进化和免疫学研究的重要模型病毒，是一种有前途的溶瘤病毒。在这项研究中,我们对MYXV病毒疫苗株B-82和Rabbivac-B的两个完整基因组进行了测序和分析，在俄罗斯广泛用于疫苗生产。这里,我们首先证明MYXV疫苗株B-82和Rabbivac-B与美国重组MYXVMAV疫苗株具有共同的起源。此外,我们的数据表明，MYXVB-82和Rabbivac-B菌株在5'和3'末端包含许多与毒力MYXV洛桑菌株相同的基因。在M013L中鉴定出几种独特的遗传特征，M017L,M023和M121R基因，有助于实现疫苗株之间的高遗传分辨率。总的来说,这些发现突出了MYXV基因组中某些基因的进化灵活性,并为该病毒的分子流行病学和后续疫苗开发提供了见解.
