Abstract：
Infectious diabetic wounds present a substantial challenge, characterized by inflammation, infection, and delayed wound healing, leading to elevated morbidity and mortality rates. In this work, we developed a multifunctional lipid nanoemulsion containing quercetin, chlorine e6, and rosemary oil (QCRLNEs) for dual anti-inflammatory and antibacterial photodynamic therapy (APDT) for treating infectious diabetic wounds. The QCRLNEs exhibited spherical morphology with a size of 51 nm with enhanced encapsulation efficiency, skin permeation, and localized delivery at the infected wound site. QCRLNEs with NIR irradiation have shown excellent wound closure and antimicrobial properties in vitro, mitigating the nonselective cytotoxic behavior of PDT. Also, excellent biocompatibility and anti-inflammatory and wound healing responses were observed in zebrafish models. The infected wound healing properties in S. aureus-infected diabetic rat models indicated re-epithelization and collagen deposition with no signs of inflammation. This multifaceted approach using QCRLNEs with NIR irradiation holds great promise for effectively combating oxidative stress and bacterial infections commonly associated with infected diabetic wounds, facilitating enhanced wound healing and improved clinical outcomes.
摘要：
感染性糖尿病伤口提出了重大挑战，以炎症为特征，感染,和延迟的伤口愈合，导致发病率和死亡率升高。在这项工作中,我们开发了一种含有槲皮素的多功能脂质纳米乳液，氯e6和迷迭香油（QCRLNEs）用于双重抗炎和抗菌光动力疗法（APDT），用于治疗感染性糖尿病伤口。QCRLNE表现出51nm大小的球形形态，具有增强的封装效率，皮肤渗透，并在受感染的伤口部位进行局部递送。具有NIR辐射的QCRLNE在体外显示出优异的伤口闭合和抗菌性能，减轻PDT的非选择性细胞毒性行为。此外，在斑马鱼模型中观察到优异的生物相容性以及抗炎和伤口愈合反应。在金黄色葡萄球菌感染的糖尿病大鼠模型中，感染的伤口愈合特性表明上皮重新形成和胶原蛋白沉积，没有炎症迹象。这种使用QCRLNE和NIR照射的多方面方法对于有效对抗氧化应激和通常与感染的糖尿病伤口相关的细菌感染具有巨大的前景。促进伤口愈合和改善临床结果。
