Abstract：
Oral ulcers, superficial lesions on the surface of the oral mucosa, have a high incidence rate, and their main symptoms include local pain and erosion. Lipopolysaccharide (LPS)-preconditioned bone marrow mesenchymal stem cells and their secreted exosomes (LPS-pre-Exos) have been shown to promote recovery in various inflammatory conditions and wounds. However, studies documenting LPS-pre-Exos as a therapeutic intervention for oral mucosal-like diseases are lacking. In this study, we prepared a silk fibroin microneedle (MN) patch consisting of LPS-pre-Exos and zeolitic imidazolate framework-8 (ZIF-8) that localized at the tip and base, respectively, and used this MN patch for oral ulcer treatment. Upon insertion into the oral mucosa, continuous LPS-pre-Exos release was observed, which promoted macrophage polarization and tissue healing. Additionally, the ZIF-8 framework in the MN patch facilitated the controlled release of Zn2+, which demonstrated potent antimicrobial properties via synergistic effects. The in vitro experimental results showed that the silk fibroin MN patch can continuously release LPS-pre-Exos and Zn2+ for more than 7 days. Thus, the LPS-pre-Exos and ZIF-8-loaded silk fibroin MN patch exhibited good anti-inflammatory and antibacterial properties, promoting oral ulcer healing, and showed good histocompatibility. Hence, it may represent a potentially valuable strategy for facilitating oral ulcer healing.
摘要：
口腔溃疡,口腔粘膜表面的浅表病变，发病率很高，其主要症状包括局部疼痛和糜烂。脂多糖（LPS）预处理的骨髓间充质干细胞及其分泌的外泌体（LPS-pre-Exos）已显示可促进各种炎症和伤口的恢复。然而,缺乏记录LPS-pre-Exos作为口腔粘膜样疾病治疗干预措施的研究。在这项研究中,我们制备了由LPS-pre-Exos和位于尖端和基部的沸石咪唑酯框架-8（ZIF-8）组成的丝素蛋白微针（MN）贴片，分别,并使用这种MN贴片治疗口腔溃疡。插入口腔粘膜后，观察到持续的LPS-Exos释放，促进巨噬细胞极化和组织愈合。此外,MN贴片中的ZIF-8框架促进了Zn2+的控释，通过协同效应证明了有效的抗菌性能。体外实验结果表明，丝素蛋白MN贴片可持续释放LPS-pre-Exos和Zn2+超过7天。因此，LPS-pre-Exos和ZIF-8负载丝素蛋白MN贴片表现出良好的抗炎和抗菌性能，促进口腔溃疡愈合，并显示出良好的组织相容性。因此，它可能是促进口腔溃疡愈合的潜在有价值的策略。
