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Abstract：
Introduction Dermatophytosis is a common infection of the skin, hair, and nails caused by dermatophytes, a group of filamentous fungi capable of digesting and obtaining nutrients from keratin. Dermatophytes comprise three important genera: Epidermophyton, Microsporum,and Trichophyton. This study aimed to analyze the antifungal susceptibility patterns of Trichophyton mentagrophytes isolates using the epsilometer test (E-test) method. Material and methods This prospective observational study was conducted on clinically suspected cases of dermatophytosis. All samples, including skin scrapings, hair, and nails, were subjected to potassium hydroxide (KOH) examination followed by fungal culture. The Trichophyton mentagrophytes isolates were then subjected to antifungal susceptibility testing using the E-test method for the two most prescribed antifungals: itraconazole and fluconazole. Results In this study, one-third of the patients who tested positive for dermatophytosis belonged to the same family, with spouses being the most commonly affected. Tinea corporis was the most common clinical presentation, with Trichophyton mentagrophytes identified as the most common etiological agent. Itraconazole was more effective than fluconazole. Conclusion The current study demonstrated that antifungal susceptibility testing of dermatophytes using the E-test is easier and can be applied in routine laboratories as a screening method, serving as an alternative to broth microdilution.
摘要：
介绍皮肤癣菌病是一种常见的皮肤感染，头发,和由皮肤癣菌引起的指甲，一组能够从角蛋白中消化和获取营养的丝状真菌。皮肤癣菌包括三个重要的属：表皮木素，小孢子菌,还有毛癣菌.本研究旨在使用ε-计试验（E-test）方法分析毛癣菌的抗真菌药敏模式。材料和方法这项前瞻性观察性研究是对临床可疑的皮肤癣菌病病例进行的。所有样品,包括皮肤刮屑,头发,和指甲,进行氢氧化钾（KOH）检查，然后进行真菌培养。然后使用E-test方法对两种最常用的抗真菌药：伊曲康唑和氟康唑，对念珠菌毛癣菌分离株进行抗真菌药敏试验。结果在这项研究中，皮肤癣菌病检测呈阳性的患者中有三分之一属于同一家庭，配偶是最常见的影响。体癣是最常见的临床表现，毛癣菌被确定为最常见的病因。伊曲康唑比氟康唑更有效。结论目前的研究表明，使用E-test进行皮肤癣菌的抗真菌药敏试验更容易，可以作为一种筛查方法在常规实验室中应用。作为一种替代肉汤微量稀释。
