METHODS: In this context, we explored the anti-inflammatory potency of seagrass extract with western blot (Bio-Rad) analysis by using LPS induced RAW macrophages as in-vitro models, western blot analysis, In-silico methods using Mastero 13.0 software.
RESULTS: The anti-inflammatory activity of Seagrass was demonstrated through down regulation of Pro-inflammatory markers such as Cyclooxygenase-2, induced Nitric oxide synthase and prostaglandin E synthase-1. The results were validated by docking the phytochemical constituents of seagrass namely Isocoumarin, Hexadecanoic acid, and Cis-9 Octadecenoic acid, 1,2 Benzene dicarboxylic acid and beta-sitosterol with TNF-alpha, COX-2, iNOS and PGES-1.
CONCLUSIONS: The methanolic extract of seagrass Halophila beccarii is a potential nutraceutical agent for combating against inflammation with a significant anti-inflammatory activity.
方法:在这种情况下,我们通过使用LPS诱导的RAW巨噬细胞作为体外模型,通过蛋白质印迹(Bio-Rad)分析探索了海草提取物的抗炎效力,蛋白质印迹分析,使用Mastero13.0软件的计算机内方法。
结果:通过下调促炎标志物如环氧合酶-2、诱导的一氧化氮合酶和前列腺素E合酶-1,证明了海草的抗炎活性。通过对接海草的植物化学成分即异香豆素,十六烷酸,和Cis-9十八碳烯酸,1,2苯二羧酸和β-谷甾醇与TNF-α,COX-2、iNOS和PGES-1。
结论:海草嗜盐菌的甲醇提取物是一种潜在的抗炎症的营养制剂,具有显著的抗炎活性。