%0 Journal Article
%T Seagrass as a potential nutraceutical to decrease pro-inflammatory markers.
%A Mathakala V
%A Ullakula T
%A Palempalli UMD
%J BMC Complement Med Ther
%V 24
%N 1
%D 2024 Jul 10
%M 38987758
%F 2.838
%R 10.1186/s12906-024-04532-z
%X <B>BACKGROUND: </B>The Pro-inflammatory mediators such as prostaglandin E2, nitric oxide and TNF-α are the key players in the stimulation of the inflammatory responses. Thus, the pro-inflammatory mediators are considered to be potential targets for screening nutraceutical with anti-inflammatory activity.<BR><B>METHODS: </B>In this context, we explored the anti-inflammatory potency of seagrass extract with western blot (Bio-Rad) analysis by using LPS induced RAW macrophages as in-vitro models, western blot analysis, In-silico methods using Mastero 13.0 software.<BR><B>RESULTS: </B>The anti-inflammatory activity of Seagrass was demonstrated through down regulation of Pro-inflammatory markers such as Cyclooxygenase-2, induced Nitric oxide synthase and prostaglandin E synthase-1. The results were validated by docking the phytochemical constituents of seagrass namely Isocoumarin, Hexadecanoic acid, and Cis-9 Octadecenoic acid, 1,2 Benzene dicarboxylic acid and beta-sitosterol with TNF-alpha, COX-2, iNOS and PGES-1.<BR><B>CONCLUSIONS: </B>The methanolic extract of seagrass Halophila beccarii is a potential nutraceutical agent for combating against inflammation with a significant anti-inflammatory activity.