PDF(Pubmed)
Abstract：
Sugars will be eventually effluxed transporters (SWEETs) have been confirmed to play diverse physiological roles in plant growth, development and stress response. However, the characteristics and functions of the SWEET genes in Hemerocallis citrina remain unclear and poorly elucidated. In this study, the whole genome of Hemerocallis citrina was utilized to conduct bioinformatics analysis and a total of 19 HcSWEET genes were successfully identified. Analysis of the physicochemical properties indicated dominant differences among these HcSWEETs. A phylogenetic analysis revealed that HcSWEET proteins can be divided into 4 clades ranging from Clade I to IV, where proteins within the same clade exhibited shared conserved motifs and gene structures. Five to six exons were contained in the majority of HcSWEET genes, which were unevenly distributed across 11 chromosomes. The gene duplication analysis showed the presence of 4 gene pairs. Comparative syntenic maps revealed that the HcSWEET gene family might present more closed homology in monocotyledons than dicotyledons. Cis-acting element analysis of HcSWEET genes indicated key responsiveness to various hormones, light, and stresses. Additionally, transcriptome sequencing analysis suggested that most HcSWEET genes had a relatively higher expression in roots, and HcSWEET4a was significantly up-regulated under salt stress. Overexpression further verified the possibility that HcSWEET4a was involved in response to salt stress, which provides novel insights and facilitates in-depth studies of the functional analysis of HcSWEETs in resistance to abiotic stress.
摘要：
糖最终将外溢的转运蛋白（SWEET）已被证实在植物生长中起着不同的生理作用，发育和应激反应。然而,Hemerocalliscitrina中SWEET基因的特征和功能仍不清楚，也不清楚。在这项研究中,利用全基因组进行生物信息学分析，成功鉴定出19个HcSWEET基因。物理化学性质的分析表明这些HcSWEET之间存在主要差异。系统发育分析表明，HcSWEET蛋白可以分为4个进化枝，从进化枝I到IV，其中同一进化枝内的蛋白质表现出共享的保守基序和基因结构。大多数HcSWEET基因中含有5到6个外显子，它们在11条染色体上分布不均。基因重复分析显示存在4个基因对。比较性图谱显示，与双子叶植物相比，HcSWEET基因家族在单子叶植物中可能表现出更多的封闭同源性。HcSWEET基因的顺式作用元件分析表明对各种激素的关键反应，光,和压力。此外,转录组测序分析表明，大多数HcSWEET基因在根中的表达相对较高，HcSWEET4a在盐胁迫下显著上调。过表达进一步证实了HcSWEET4a参与盐胁迫反应的可能性,这提供了新颖的见解，并促进了对HcSWEET在抵抗非生物胁迫方面的功能分析的深入研究。
