Abstract：
Barrier membranes play a prominent role in guided bone regeneration (GBR), and polycaprolactone (PCL) is an attractive biomaterial for the fabrication of barrier membranes. However, these nanofiber membranes (NFMs) require modification to improve their biological activity. PCL-NFMs incorporating with laponite (LAP) achieve biofunctional modification. Decellularized extracellular matrix (dECM) could modulate cell behaviour. The present study combined dECM with PCL/LAP-NFMs to generate a promising strategy for bone tissue regeneration. Bone marrow mesenchymal stem cells (BMSCs) were cultured on NFMs and deposited with an abundant extracellular matrix (ECM), which was subsequently decellularized to obtain dECM-modified PCL/LAP-NFMs (PCL/LAP-dECM-NFMs). The biological functions of the membranes were evaluated by reseeding MC3T3-E1 cells in vitro and transplanting them into rat calvarial defects in vivo. These results indicate that PCL/LAP-dECM-NFMs were successfully constructed. The presence of dECM slightly improved the mechanical properties of the NFMs, which exhibited a Young\'s modulus of 0.269 MPa, ultimate tensile strength of 2.04 MPa and elongation at break of 51.62 %. In vitro, the PCL/LAP-dECM-NFMs had favourable cytocompatibility, and the enhanced hydrophilicity was conducive to cell adhesion, proliferation, and osteoblast differentiation. PCL/LAP-dECM-NFMs exhibited an excellent bone repair capacity in vivo. Overall, dECM-modified PCL/LAP-NFMs should be promising biomimetic barrier membranes for GBR.
摘要：
屏障膜在引导骨再生（GBR）中起着重要作用，聚己内酯(PCL)是用于制造屏障膜的有吸引力的生物材料。然而,这些纳米纤维膜(NFM)需要修饰以提高其生物活性。与锂皂石(LAP)结合的PCL-NFM实现生物功能改性。脱细胞细胞外基质（dECM）可以调节细胞行为。本研究将dECM与PCL/LAP-NFM相结合，为骨组织再生提供了一种有希望的策略。骨髓间充质干细胞（BMSCs）在NFM上培养，并沉积有丰富的细胞外基质（ECM），随后将其去细胞化以获得dECM修饰的PCL/LAP-NFM(PCL/LAP-dECM-NFM)。通过在体外重新接种MC3T3-E1细胞并将其移植到体内大鼠颅骨缺损中，评估了膜的生物学功能。这些结果表明成功构建了PCL/LAP-dECM-NFM。dECM的存在略微改善了NFM的机械性能，其杨氏模量为0.269MPa，极限拉伸强度为2.04MPa，断裂伸长率为51.62%。体外，PCL/LAP-dECM-NFM具有良好的细胞相容性，增强的亲水性有利于细胞粘附，扩散,和成骨细胞分化。PCL/LAP-dECM-NFM在体内表现出优异的骨修复能力。总的来说,dECM修饰的PCL/LAP-NFM应该是有前途的GBR仿生屏障膜。
