Abstract：
The extremely slow growth rate of anaerobic ammonia oxidation (anammox) bacteria limits full-scale application of anammox process worldwide. In this study, extracellular polymeric substances (EPS)-coated polypropylene (PP) carriers were prepared for biofilm formation. The biomass adhesion rate of EPS-PP carrier was 12 times that of PP carrier, and EPS-PP achieved significant enrichment of E. coli BY63. The 120-day continuous flow experiment showed that the EPS-PP carrier accelerated the formation of anammox biofilm, and the nitrogen removal efficiency increased by 10.5 %. In addition, the abundance of Candidatus Kuenenia in EPS-PP biofilm was 27.1%. Simultaneously, amino acids with high synthesis cost and the metabolites of glycerophospholipids related to biofilm formation on EPS-PP biofilm were significantly up-regulated. Therefore, EPS-PP carriers facilitated the rapid formation of anammox biofilm and promoted the metabolic activity of functional bacteria, which further contributed to the environmental and economic sustainability of anammox process.
摘要：
厌氧氨氧化(anammox)细菌的极其缓慢的生长速率限制了anammox工艺在世界范围内的全面应用。在这项研究中,制备胞外聚合物（EPS）包覆的聚丙烯（PP）载体用于生物膜形成。EPS-PP载体的生物质附着率为PP载体的12倍,EPS-PP实现了大肠杆菌BY63的显著富集。120天连续流实验表明，EPS-PP载体加速了厌氧氨氧化生物膜的形成，脱氮效率提高了10.5%。此外,EPS-PP生物膜中Kuenenia念珠菌的丰度为27.1%。同时,EPS-PP生物膜上合成成本高的氨基酸和与生物膜形成相关的甘油磷脂代谢产物显著上调。因此,EPS-PP载体促进厌氧氨氧化生物膜的快速形成,促进功能菌的代谢活性,这进一步促进了厌氧氨氧化过程的环境和经济可持续性。
