Abstract：
Developing extracellular matrix-derived hydrogel with a fast self-healing capacity to provide a sustainable moist environment able to accelerate wound healing is highly desired for full-thickness skin wound repair. In this study, a fast self-healing hyaluronic acid hydrogel with a dual dynamic network was constructed through a primary reversible acylhydrazone bond formed between aldehyde-modified hyaluronic acid, 3,3\'-dithiobis (propionyl hydrazide) (DTP), and secondary dynamic ionic interactions between κ-carrageenan (KC) and K+. Because of the presence of various dynamic covalent bonds such as the acylhydrazone bond, disulfide bond, and noncovalent bonds including hydrogen bonding and ionic interactions, as well as the notable thermoreversible nature of KC, the resultant hydrogel could be self-healed rapidly within 30 min under physiological temperature with a self-healing efficiency of 100%, which was significantly better than other hyaluronic acid hydrogels, as reported previously. Besides, the hydrogel displayed excellent cytocompatibility. According to this study, the hydrogel was administered into the wounds and achieved a superior performance of promoting full-thickness skin wound healing by increasing granulation tissue formation, deposition of collagen as well as the acceleration of re-epithelialization and neovascularization, compared to commercial products, e.g., gauze and 3 M hydrocolloid. We also anticipate that this strategy of double-dynamic network cross-linking can be adopted to fabricate self-healing materials for multiple applications.
摘要：
开发具有快速自修复能力的细胞外基质衍生的水凝胶以提供能够加速伤口愈合的可持续潮湿环境对于全厚度皮肤伤口修复是高度期望的。在这项研究中,通过醛改性透明质酸之间形成的主要可逆酰腙键，构建了具有双重动态网络的快速自修复透明质酸水凝胶，3,3'-二硫代双（丙酰肼）（DTP），以及κ-角叉菜胶（KC）和K之间的二次动态离子相互作用。由于各种动态共价键如酰腙键的存在，二硫键,和非共价键，包括氢键和离子相互作用，以及KC显着的热可逆性质，得到的水凝胶在生理温度下30min内可快速自愈,自愈效率为100%,明显优于其他透明质酸水凝胶，正如之前报道的。此外,水凝胶显示出优异的细胞相容性。根据这项研究,将水凝胶施用到伤口中，并通过增加肉芽组织的形成来实现促进全层皮肤伤口愈合的优异性能，胶原蛋白的沉积以及上皮再生和新生血管的加速，与商业产品相比，例如，纱布和3M水胶体。我们还预计这种双动态网络交联的策略可以用于制造用于多种应用的自修复材料。
