Abstract：
Microplastics is known to be ubiquitous in aquatic environment. Quantification of microplastics in natural waters is an important problem of analytical chemistry, the solution of which is needed for the assessment of water quality and potential risks for water inhabitants and consumers. Separation methods play a key role in the correct quantification of microplastics in natural waters. In the present study the applicability of countercurrent chromatography to the continuous-flow separation and preconcentration of microplastics from water samples in rotating coiled column (RCC) using water-oil systems has been demonstrated for the first time. The effect of column rotation speed and mobile phase (water) flow rate on the retention of the stationary (oil) phase in RCC is studied. The retention parameters of 10 vegetable and 2 synthetic oils are determined. Castor, olive, rapeseed, soybean, linseed, sesame, and sunflower oils are found to be applicable to the separation of microplastics from water samples using RCC. Taking as example polyethylene microparticles of different size (40-63, 63-100, and 100-250 μm), the high recovery of microplastics (about 100 %) from aqueous phase into castor and rapeseed oils is shown. The method has been proven to be efficient for the separation of microplastics from simulated fresh and sea natural waters. It may be perspective not only for the quantification of microplastics in natural waters but as well as for the purification of wastewaters containing microplastics.
摘要：
已知微塑料在水生环境中无处不在。天然水中微塑料的定量是分析化学的一个重要问题，评估水质和水居民和消费者的潜在风险是需要的解决方案。分离方法在正确定量天然水中的微塑料中起着关键作用。在本研究中，首次证明了逆流色谱法在使用水-油系统的旋转盘绕柱（RCC）中从水样中连续流分离和预浓缩微塑料的适用性。研究了色谱柱转速和流动相（水）流速对RCC中固定（油）相保留的影响。确定了10种植物油和2种合成油的保留参数。蓖麻,橄榄,油菜籽,大豆,亚麻籽,芝麻,发现向日葵油适用于使用RCC从水样中分离微塑料。以不同尺寸(40-63、63-100和100-250μm)的聚乙烯微粒为例，显示出微塑料从水相中到蓖麻油和菜籽油中的高回收率（约100％）。该方法已被证明可有效地从模拟的淡水和海水中分离微塑料。它不仅可以用于量化天然水中的微塑料，而且可以用于净化含有微塑料的废水。
