Abstract：
Jackfruit leaf protein hydrolysates obtained from the enzymatic hydrolysis of leaf protein concentrate with gastrointestinal enzymes have shown good techno-functional properties and high antioxidant capacity. However, molecular weight, antiproliferative activity, cytotoxicity and the ability to reduce reactive oxygen species (ROS) are still unknown. Therefore, this study aimed to evaluate the effect of jackfruit leaf protein hydrolysates obtained by enzymatic hydrolysis with pepsin and pancreatin at different hydrolysis times (30-240 min) on molecular weights, cytotoxicity, antiproliferation of cancer cells, and the reduction of reactive oxygen species in H2O2-induced HaCaT cells. The electrophoretic profile indicated that H-Pep contains peptides with molecular weights between 25 - 20 kDa. Meanwhile, H-Pan is composed of molecular weight products between 25 - 20 kDa and < 20 kDa. H-Pan and H-Pep (125-500 µg/mL) did not show significant cytotoxicity on HaCaT (human keratinocytes) and J774A.1 (murine macrophage cells). Antiproliferative activity was achieved in human cervical, ovarian, and liver cancer cells. H-Pan-240 min (1000 µg/mL) reduced the cell viability of cervical cancer cells by 23% while H-Pan-60 min significantly reduced cell viability of ovarian and liver cancer cells by 14.5 (500 µg/mL) and 17% (1000 µg/mL), respectively (P < 0.05). The protective effect against oxidative stress on H2O2-stressed HaCaT cells was obtained with H-Pep-60 min, which reduced 25% of ROS at 250 µg/mL (P < 0.05). The findings demonstrate the safe use of green biomass as a source of plant protein hydrolysates.
摘要：
用胃肠酶酶水解叶蛋白浓缩物获得的菠萝蜜叶蛋白水解产物具有良好的技术功能特性和较高的抗氧化能力。然而,分子量,抗增殖活性，细胞毒性和减少活性氧（ROS）的能力仍然未知。因此,本研究旨在评估通过胃蛋白酶和胰酶酶酶水解获得的菠萝蜜叶蛋白水解产物在不同水解时间（30-240分钟）对分子量的影响，细胞毒性，癌细胞的抗增殖，以及H2O2诱导的HaCaT细胞中活性氧的减少。电泳图谱表明H-Pep含有分子量在25-20kDa之间的肽。同时,H-Pan由分子量在25-20kDa和<20kDa之间的产物组成。H-Pan和H-Pep（125-500µg/mL）对HaCaT（人角质形成细胞）和J774A.1（鼠巨噬细胞）未显示出明显的细胞毒性。在人宫颈中实现了抗增殖活性，卵巢,和肝癌细胞。H-Pan-240分钟（1000µg/mL）将宫颈癌细胞的细胞活力降低了23％，而H-Pan-60分钟则显着降低了卵巢癌和肝癌细胞的细胞活力14.5（500µg/mL）和17％（1000µg/mL），分别为(P<0.05)。H-Pep-60min对H2O2胁迫的HaCaT细胞具有抗氧化应激的保护作用，在250µg/mL时减少25%的ROS（P<0.05）。研究结果表明，绿色生物质可安全用作植物蛋白水解产物的来源。
