Abstract:
The filamentous bacteriophage M13KO7 (M13) is the most used in phage display (PD) technology and, like other phages, has been applied in several areas of medicine, agriculture, and in the food industry. One of the advantages is that they can modulate the immune response in the presence of pathogenic microorganisms, such as bacteria and viruses. This study evaluated the use of phage M13 in the chicken embryos model. We inoculated 13-day-old chicken embryos with Salmonella Pullorum (SP) and then evaluated survival for the presence of phage M13 or E. coli ER2738 (ECR) infected with M13. We found that the ECR bacterium inhibits SP multiplication in 0.32 (M13-infected ECR) or 0.44 log UFC/mL (M13-uninfected ECR) and that the ECR-free phage M13 from the PD library can be used in chicken embryo models. This work provides the use of the chicken embryo as a model to study systemic infection and can be employed as an analysis tool for various peptides that M13 can express from PD selection. KEY POINTS: • SP-infected chicken embryo can be a helpful model of systemic infection for different tests. • Phage M13 does not lead to embryonic mortality or cause serious injury to embryos. • Phage M13 from the PD library can be used in chicken embryo model tests.
摘要:
丝状噬菌体M13KO7(M13)是噬菌体展示(PD)技术中最常用的噬菌体,像其他噬菌体一样,已被应用于多个医学领域,农业,在食品工业中。优点之一是它们可以在病原微生物的存在下调节免疫反应,如细菌和病毒。本研讨评价了噬菌体M13在鸡胚模子中的运用。我们用沙门氏菌(SP)接种13天大的鸡胚,然后评估是否存在噬菌体M13或感染M13的大肠杆菌ER2738(ECR)的存活率。我们发现ECR细菌以0.32(M13感染的ECR)或0.44logUFC/mL(M13未感染的ECR)抑制SP增殖,并且PD文库中无ECR的噬菌体M13可用于鸡胚模型。这项工作提供了鸡胚作为研究全身性感染的模型的用途,并且可以用作M13可以从PD选择中表达的各种肽的分析工具。关键点:•SP感染的鸡胚可以是用于不同测试的系统感染的有用模型。噬菌体M13不会导致胚胎死亡或对胚胎造成严重伤害。•来自PD库的噬菌体M13可用于鸡胚模型试验。