{Reference Type}: Journal Article
{Title}: A new standardization for the use of chicken embryo: selection of target from the phage display library and infection.
{Author}: de Souza JB;Sommerfeld S;Almeida-Souza HO;Vaz ER;Bastos LM;Santos FAA;Rodrigues AC;Medeiros-Ronchi AA;Goulart LR;Fonseca BB;
{Journal}: Appl Microbiol Biotechnol
{Volume}: 108
{Issue}: 1
{Year}: 2024 Jul 10
{Factor}: 5.56
{DOI}: 10.1007/s00253-024-13227-x
{Abstract}: The filamentous bacteriophage M13KO7 (M13) is the most used in phage display (PD) technology and, like other phages, has been applied in several areas of medicine, agriculture, and in the food industry. One of the advantages is that they can modulate the immune response in the presence of pathogenic microorganisms, such as bacteria and viruses. This study evaluated the use of phage M13 in the chicken embryos model. We inoculated 13-day-old chicken embryos with Salmonella Pullorum (SP) and then evaluated survival for the presence of phage M13 or E. coli ER2738 (ECR) infected with M13. We found that the ECR bacterium inhibits SP multiplication in 0.32 (M13-infected ECR) or 0.44 log UFC/mL (M13-uninfected ECR) and that the ECR-free phage M13 from the PD library can be used in chicken embryo models. This work provides the use of the chicken embryo as a model to study systemic infection and can be employed as an analysis tool for various peptides that M13 can express from PD selection. KEY POINTS: • SP-infected chicken embryo can be a helpful model of systemic infection for different tests. • Phage M13 does not lead to embryonic mortality or cause serious injury to embryos. • Phage M13 from the PD library can be used in chicken embryo model tests.