Abstract：
Abiotic environmental stressors cause various types of damage to plants and cause significant loss in yield. Abiotic stress tolerance in plants refers to the ability to withstand environmental factors and maintain growth, development, and production. Since this tolerance is controlled by a gene or a set of genes, transgenic activating of these genes in plants often enhances tolerance under abiotic stress. Therefore, this methodology chapter describes a strategy and the corresponding protocols needed to induce a gene by an abiotic stressor, clone the corresponding cDNA into plasmids and Agrobacterium cells, and genetic transformation to the Arabidopsis plants using the floral dip method. The chapter also describes standard assays to evaluate the transgene\'s effect on the plant\'s tolerance. Finally, the techniques outlined in this chapter for cloning and generating transgenic plants tolerant to abiotic stress are a versatile approach that can be implemented across various plant species and genes.
摘要：
非生物环境胁迫会对植物造成各种类型的损害，并导致产量的重大损失。植物中的非生物胁迫耐受性是指抵抗环境因素和维持生长的能力，发展,和生产。由于这种耐受性是由一个基因或一组基因控制的，这些基因在植物中的转基因激活通常会增强非生物胁迫下的耐受性。因此,本方法学章节描述了非生物应激源诱导基因所需的策略和相应的方案，将相应的cDNA克隆到质粒和农杆菌细胞中，并使用花浸方法对拟南芥植物进行遗传转化。本章还描述了评估转基因对植物耐受性影响的标准试验。最后,本章中概述的用于克隆和产生耐受非生物胁迫的转基因植物的技术是一种通用的方法，可以在各种植物物种和基因中实施。
