Abstract：
Chloroplast isolation protocols have been extensively developed for various species of plants, particularly model organisms with easily manipulable physical characteristics. However, succulent plants, such as Agave angustifolia Haw., which possess adaptations for arid environments like the Crassulacean acid metabolism (CAM) and a thicker cuticle, have received less attention, resulting in a potential knowledge gap. This chapter presents a specialized protocol focusing on isolating chloroplast from A. angustifolia, a species exhibiting adaptations to arid conditions and holding ecological and economic significance due to its role in producing bacanora and mezcal beverages. By successfully isolating chloroplast from A. angustifolia plant growth in ex vitro and in vitro conditions, this protocol enables comprehensive future analyses to elucidate metabolic processes and explore potential applications in related species. Consequently, this research aims to bridge this knowledge gap in chloroplast isolation for succulent plants, providing new insights for future investigations in the field.
摘要：
叶绿体分离方案已广泛用于各种植物物种，特别是具有易于操作的物理特征的模型生物。然而,多肉植物,如龙舌兰。,具有对干旱环境的适应性，如Crassulacean酸代谢（CAM）和较厚的角质层，受到的关注较少，导致潜在的知识差距。本章介绍了一个专门的方案，重点是从A.angustifolia中分离叶绿体，由于其在生产bacanora和mezcal饮料中的作用，对干旱条件具有适应性并具有生态和经济意义的物种。通过在体外和体外条件下成功分离出生长的沙棘植物叶绿体，该协议可以进行全面的未来分析，以阐明代谢过程并探索相关物种的潜在应用。因此,这项研究旨在弥合多肉植物叶绿体分离的知识差距，为该领域未来的调查提供新的见解。
