Abstract：
This chapter presents an efficient protocol for regenerating Carica papaya plants via somatic embryogenesis from immature zygotic embryos from economically important papaya genotypes. To achieve regenerated plants from somatic embryos, in the present protocol, four induction cycles are required, followed by one multiplication cycle and one regeneration cycle. With this optimized protocol, 80% of somatic embryos can be obtained in only 3.5 months. At this stage, calli containing more than 50% globular structures can be used for transformation (via agrobacterium, biobalistics, or any other transformation method). Once transformed, calli can be transferred to the following steps (multiplication, elongation, maturation, rooting, and ex vitro acclimatization) to regenerate a transformed somatic embryo-derived full plant.
摘要：
本章提出了一种有效的方案，用于通过从经济上重要的木瓜基因型的未成熟合子胚中通过体细胞胚发生再生番木瓜植物。为了从体细胞胚获得再生植物，在本协议中，需要四个感应周期，随后是一个增殖周期和一个再生周期。有了这个优化的协议，80％的体细胞胚可以在3.5个月内获得。在这个阶段,含有50%以上球状结构的愈伤组织可用于转化(通过农杆菌，生物物理学，或任何其他转换方法)。一旦转化，愈伤组织可以转移到以下步骤(乘法，伸长率,成熟,生根,和体外适应）以再生转化的体细胞胚来源的完整植物。
