Abstract：
This chapter presents the methodological approach for the in vitro propagation of Agave angustifolia \"espadin,\" the base material to produce mezcal. The protocol used in each stage of the crop is addressed in detail, considering the changes in the culture medium and the characteristics of the plant material at each stage. The importance of integrated management between the multiplication and growth phase, as part of the in vitro selection strategy, is mentioned.
摘要：
本章介绍了龙葵离体繁殖的方法学方法，“生产培养基的基础材料。在作物的每个阶段使用的协议都有详细说明，考虑培养基的变化和植物材料在每个阶段的特性。在增殖和成长阶段之间进行综合管理的重要性，作为体外选择策略的一部分，提到了。
