Abstract：
Temporary immersion systems (TIS) have been widely recognized as a promising technology for micropropagation of various plant species. The TIS provides a suitable environment for culture and allows intermittent contact of the explant with the culture medium at different immersion frequencies and aeration of the culture in each cycle. The frequency or immersion is one of the most critical parameters for the efficiency of these systems. The design, media volume, and container capacity substantially improve cultivation efficiency. Different TIS have been developed and successfully applied to micropropagation in various in vitro systems, such as sprout proliferation, microcuttings, and somatic embryos. TIS increases multiplication and conversion rates to plants and a better response during the ex vitro acclimatization phase. This article covers the use of different immersion systems and their applications in plant biotechnology, particularly in plant tissue culture, as well as its use in the massive propagation of plants of agroeconomic interest.
摘要：
临时浸没系统（TIS）已被广泛认为是用于各种植物物种的微繁殖的有前途的技术。TIS为培养提供了合适的环境，并允许外植体与培养基以不同的浸入频率间歇接触，并在每个循环中进行培养。频率或浸入是这些系统效率的最关键参数之一。设计，媒体卷,和容器容量大大提高了栽培效率。已经开发了不同的TIS，并成功地将其应用于各种体外系统中的微繁殖，如发芽增殖，微插条,和体细胞胚胎。TIS增加了对植物的增殖和转化率，并在体外适应阶段获得了更好的响应。本文介绍了不同浸没系统的使用及其在植物生物技术中的应用，特别是在植物组织培养中，以及它在农业经济利益植物的大规模繁殖中的用途。
