PDF(Pubmed)
Abstract：
The purpose of this study was to evaluate how ascorbic acid with dietary flaxseed oil affects the quality and fertility of cryopreserved ram sperm in South African indigenous rams. Treatment diets were supplemented 60 days before semen collection to afford proper spermatogenesis, adaptation to the feed formulated and fed throughout the study. Semen was collected with the use of artificial vagina following dietary supplementation with five treatment diets (neg. cont. - negative control, pos. cont. - positive control, FLO - 5% Flaxseed oil, ASA - 4% Ascorbic acid, and FLO + ASA). Semen was then extended using tris-based extender and cryopreserved using the programmable freezer (CBS Freezer 2100 series, Laboratory consumables & chemical suppliers, America). Ovaries were collected from a neighbouring slaughter house and conveyed to the lab in 0.9% saline at 37 °C. Data (sperm parameters and in vitro fertility) was then exposed to the GLM (General Linear Model) in Minitab 17. Pearson\'s correlation coefficient was utilized to investigate the relationship between cryopreserved sperm quality and in vitro fertility. The student Least Significant Difference Test was used to separate the treatment means, and differences were accepted when the p-value was less than 0.05. The FLO + ASA group had higher (p < 0.05) progressive (36.33 ± 1.87), total (88.24 ± 2.24), rapid motility (27.52 ± 1.74), intact plasma membrane (75.67 ± 2.08), total fertilization (65.98 ± 7.39), and total cleavage (66.19 ± 6.50) when compared to other treatment groups. Total fertilization rate had a medium significant (p < 0.001) medium correlation with the progressive motility (r2 = 0.435), total motility (r2 = 0.447) and rapid motility (r2 = 0.409). In conclusion, dietary flaxseed and ascorbic acid (FLO + ASA) improves cryopreserved semen quality, in vitro fertilization rate, and the total cleavage rate. Noteworthy, the progressive, total and rapid motility play a crucial in the in vitro fertilization rate.
摘要：
这项研究的目的是评估饮食亚麻籽油中的抗坏血酸如何影响南非本地公羊冷冻保存的公羊精子的质量和生育力。在收集精液前60天补充治疗饮食，以提供适当的精子发生，在整个研究过程中适应配制和喂养的饲料。在饮食补充五种治疗饮食（neg。cont.-阴性对照，pos.cont.-阳性对照，FLO-5%亚麻籽油,ASA-4%抗坏血酸，和FLO+ASA)。然后使用基于Tris的扩展器扩展精液，并使用可编程冷冻机（CBS冷冻机2100系列，实验室耗材和化学品供应商，美国）。从邻近的屠宰场收集卵巢，并在37°C的0.9%盐水中输送到实验室。然后将数据(精子参数和体外生育力)暴露于Minitab17中的GLM(一般线性模型)。利用Pearson相关系数研究冻存精子质量与体外受精的关系。学生最小显著差异测试用于分离治疗手段，当p值小于0.05时，差异被接受。FLO+ASA组有更高的进展(p<0.05)(36.33±1.87),总计（88.24±2.24），快速运动（27.52±1.74），完整的质膜（75.67±2.08），总施肥（65.98±7.39），和总切割（66.19±6.50）当与其他治疗组相比。总受精率与渐进运动性(r2=0.435)有中等显著的(p<0.001)相关性，总运动性（r2=0.447）和快速运动性（r2=0.409）。总之,膳食亚麻籽和抗坏血酸（FLO+ASA）提高冷冻保存的精液质量，体外受精率，和总卵裂率。值得注意的是,进步,总体和快速运动在体外受精率中起着至关重要的作用。
