Abstract:
A novel Gram-negative, white-pigmented, and auxin-producing strain, 20NA77.5T, was isolated from fresh water during cyanobacterial bloom period. Pairwise comparison of the 16S rRNA gene sequences showed that strain 20NA77.5T belonged to the genus Undibacterium and exhibited the highest sequence similarity to the type strains of Undibacterium danionis (98.00%), Undibacterium baiyunense (97.93%), Undibacterium macrobrachii (97.92%), and Undibacterium fentianense (97.71%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain 20NA77.5T and its related type strains were below 79.93 and 23.80%, respectively. The predominant fatty acids (> 10% of the total fatty acids) were C16:0 and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The genomic DNA G + C content of strain 20NA77.5T was found to be 48.61%. Based on the phylogenetic distinctness, chemotaxonomic features, and phenotypic features, strain 20NA77.5T is considered to represent a novel species of the genus Undibacterium, for which the name Undibacterium cyanobacteriorum sp. nov is proposed. The type strain is 20NA77.5T (= KCTC 8005T = LMG 33136T).
摘要:
一个新的革兰氏阴性,白色颜料,和生长素产生菌株,20NA77.5T,在蓝藻水华期间从淡水中分离出来。对16SrRNA基因序列的成对比较表明,菌株20NA77.5T属于Undibacterium属,与danionisUndibacterium类型菌株的序列相似性最高(98.00%),白云非二杆菌(97.93%),大布氏杆菌(97.92%),和芬氏杆菌(97.71%)。20NA77.5T菌株及其相关类型菌株之间的平均核苷酸同一性(ANI)和数字DNA-DNA杂交(dDDH)值低于79.93和23.80%,分别。主要的脂肪酸(>10%的总脂肪酸)是C16:0和总计特征3(C16:1ω7c和/或C16:1ω6c)。发现菌株20NA77.5T的基因组DNAGC含量为48.61%。基于系统发育的差异,化学分类学特征,和表型特征,菌株20NA77.5T被认为代表了Undibacterium属的一种新物种,其名称为蓝藻Undibacteriumsp.nov被提议。菌株类型为20NA77.5T(=KCTC8005T=LMG33136T)。