Abstract：
Producers of fish have been looking for viable alternatives for the management of Colossoma macropomum (tambaqui) in confinement systems in order to avoid the harm and subsequent losses caused by parasitic diseases. One alternative used by farmers is pesticides, such as trichlorfon, which has a genotoxic effect. Thus, this study aimed to evaluate the changes in gene expression due to the side effects of trichlorfon in tambaqui. Two treatments were used based on LC50-96h of 0.870 mg/L using 30% and 50% trichlorfon with exposure periods of 48, 72 and 96 h. For differential expression of the genes in the liver, real-time PCR was performed for the AChE, GST, CYP2J6, CYP2C8, 18S and GAPDH genes. After 96 h of exposure to trichlorfon, an alteration in the gene expression profile of the antioxidant defense system (GST) of the tambaqui was observed. It was also observed that this organophosphate did not affect the expression of genes related to the isoenzymes that are responsible for the biotransformation of xenobiotics in phase I (2J6 and 2C8) and cholinesterase AChE. It was concluded that the reduction in gene expression of GST suggests a decrease in metabolization capacity in phase II.
摘要：
鱼类生产者一直在寻找可行的替代方法来管理禁闭系统中的大型巨目（tambaqui），以避免寄生虫病造成的伤害和随后的损失。农民使用的一种替代品是杀虫剂，比如敌百虫，有基因毒性作用.因此，本研究旨在评估敌百虫在tambaqui中的副作用引起的基因表达变化。使用两种处理基于0.870mg/L的LC50-96h，使用30％和50％敌百虫，暴露期为48、72和96h。对于肝脏中基因的差异表达，对AChE进行实时PCR，GST,CYP2J6、CYP2C8、18S和GAPDH基因。接触敌百虫96小时后，观察到tambaqui的抗氧化防御系统（GST）的基因表达谱发生了变化。还观察到，这种有机磷酸酯不会影响与同工酶相关的基因的表达，这些同工酶负责I期（2J6和2C8）和胆碱酯酶AChE的生物转化。结论是，GST基因表达的降低表明II期代谢能力降低。
