Abstract：
BACKGROUND: Calliptamus italicus L. is a major pest in Xinjiang grassland. The diapause overwintering strategy is one of the important reasons for the large population of this pest. This study investigated the function of the genes associated with the release of diapause (DIB, JHE and CAM) in Calliptamus italicus by RNA interference (RNAi) technology to aid in its biological control.
RESULTS: The expression levels of DIB and its downstream-associated genes (EcR and FTZ-F1) in the eggs injected with dsDIB for 12 h decreased by 96.6%, 55.8% and 81.8%, respectively. Diapause began to terminate on day 3, and development was almost complete on day 6. However, the head was significantly smaller. The expression levels of JHE and its downstream-associated genes (JHEH and VgR) at 48 h after dsJHE treatment decreased by 76.5%, 85.6% and 85.9%, respectively. The termination of diapause occured on day 3 of incubation. The development was basically complete on day 6, but the yolk had been incompletely absorbed. The expression of CAM and its downstream-associated genes (CAMK4 and MYL) at 24 h after dsCAM treatment decreased by 42.4%, 95.3% and 82.7%, respectively. Diapause termination was completed on day 4 for incubation, and development was abnormal on day 6. The absorption of yolk was incomplete.
CONCLUSIONS: DIB, JHE and CAM can delay the diapause termination of Calliptamus italicus eggs to different degrees and can be developed as potential target genes for its biological control. © 2024 Society of Chemical Industry.
RESULTS: The expression levels of DIB and its downstream-associated genes (EcR and FTZ-F1) in the eggs injected with dsDIB for 12 h decreased by 96.6%, 55.8% and 81.8%, respectively. Diapause began to terminate on day 3, and development was almost complete on day 6. However, the head was significantly smaller. The expression levels of JHE and its downstream-associated genes (JHEH and VgR) at 48 h after dsJHE treatment decreased by 76.5%, 85.6% and 85.9%, respectively. The termination of diapause occured on day 3 of incubation. The development was basically complete on day 6, but the yolk had been incompletely absorbed. The expression of CAM and its downstream-associated genes (CAMK4 and MYL) at 24 h after dsCAM treatment decreased by 42.4%, 95.3% and 82.7%, respectively. Diapause termination was completed on day 4 for incubation, and development was abnormal on day 6. The absorption of yolk was incomplete.
CONCLUSIONS: DIB, JHE and CAM can delay the diapause termination of Calliptamus italicus eggs to different degrees and can be developed as potential target genes for its biological control. © 2024 Society of Chemical Industry.
摘要：
背景：卡利巴是新疆草地的主要害虫。滞育越冬策略是该害虫种群众多的重要原因之一。这项研究调查了与滞育释放相关的基因的功能（DIB，JHE和CAM）通过RNA干扰（RNAi）技术辅助其生物防治。
结果:注射dsDIB12h的卵中DIB及其下游相关基因(EcR和FTZ-F1)的表达水平下降了96.6%,55.8%和81.8%，分别。滞育在第3天开始终止，发育在第6天几乎完成。然而,头部明显变小。dsJHE处理48h后JHE及其下游相关基因(JHEH和VgR)的表达水平下降了76.5%,85.6%和85.9%，分别。滞育的终止发生在孵育的第3天。发育在第6天基本完成,但蛋黄未被完全吸收。dsCAM处理后24hCAM及其下游相关基因(CAMK4和MYL)的表达下降了42.4%,95.3%和82.7%，分别。停育终止在第4天完成，用于孵育，第6天发育异常。卵黄的吸收不完全。
结论：DIB，JHE和CAM可以在不同程度上延迟意大利卡利巴卵的滞育终止，并可作为其生物防治的潜在靶基因。©2024化学工业学会。
结果:注射dsDIB12h的卵中DIB及其下游相关基因(EcR和FTZ-F1)的表达水平下降了96.6%,55.8%和81.8%，分别。滞育在第3天开始终止，发育在第6天几乎完成。然而,头部明显变小。dsJHE处理48h后JHE及其下游相关基因(JHEH和VgR)的表达水平下降了76.5%,85.6%和85.9%，分别。滞育的终止发生在孵育的第3天。发育在第6天基本完成,但蛋黄未被完全吸收。dsCAM处理后24hCAM及其下游相关基因(CAMK4和MYL)的表达下降了42.4%,95.3%和82.7%，分别。停育终止在第4天完成，用于孵育，第6天发育异常。卵黄的吸收不完全。
结论：DIB，JHE和CAM可以在不同程度上延迟意大利卡利巴卵的滞育终止，并可作为其生物防治的潜在靶基因。©2024化学工业学会。
