RESULTS: The expression levels of DIB and its downstream-associated genes (EcR and FTZ-F1) in the eggs injected with dsDIB for 12 h decreased by 96.6%, 55.8% and 81.8%, respectively. Diapause began to terminate on day 3, and development was almost complete on day 6. However, the head was significantly smaller. The expression levels of JHE and its downstream-associated genes (JHEH and VgR) at 48 h after dsJHE treatment decreased by 76.5%, 85.6% and 85.9%, respectively. The termination of diapause occured on day 3 of incubation. The development was basically complete on day 6, but the yolk had been incompletely absorbed. The expression of CAM and its downstream-associated genes (CAMK4 and MYL) at 24 h after dsCAM treatment decreased by 42.4%, 95.3% and 82.7%, respectively. Diapause termination was completed on day 4 for incubation, and development was abnormal on day 6. The absorption of yolk was incomplete.
CONCLUSIONS: DIB, JHE and CAM can delay the diapause termination of Calliptamus italicus eggs to different degrees and can be developed as potential target genes for its biological control. © 2024 Society of Chemical Industry.
结果:注射dsDIB12h的卵中DIB及其下游相关基因(EcR和FTZ-F1)的表达水平下降了96.6%,55.8%和81.8%,分别。滞育在第3天开始终止,发育在第6天几乎完成。然而,头部明显变小。dsJHE处理48h后JHE及其下游相关基因(JHEH和VgR)的表达水平下降了76.5%,85.6%和85.9%,分别。滞育的终止发生在孵育的第3天。发育在第6天基本完成,但蛋黄未被完全吸收。dsCAM处理后24hCAM及其下游相关基因(CAMK4和MYL)的表达下降了42.4%,95.3%和82.7%,分别。停育终止在第4天完成,用于孵育,第6天发育异常。卵黄的吸收不完全。
结论:DIB,JHE和CAM可以在不同程度上延迟意大利卡利巴卵的滞育终止,并可作为其生物防治的潜在靶基因。©2024化学工业学会。