PDF(Pubmed)
Abstract：
Rapid analysis of multiple food allergens is required to confirm the appropriateness of food allergen labelling in processed foods. This study aimed to develop a rapid and reliable method to simultaneously detect trace amounts of seven food allergenic proteins (wheat, buckwheat, milk, egg, crustacean, peanut, and walnut) in processed foods using LC-MS/MS. Suspension-trapping (S-Trap) columns and on-line automated solid-phase extraction were used to improve the complex and time-consuming pretreatment process previously required for allergen analysis using LC-MS/MS. The developed method enabled the simultaneous detection of selected marker peptides for specific proteins derived from seven food ingredients in five types of incurred samples amended with trace amounts of allergenic proteins. The limit of detection values of the method for each protein were estimated to be <1 mg/kg. The developed analytical approach is considered an effective screening method for confirming food allergen labelling on a wide range of processed foods.
摘要：
需要对多种食物过敏原进行快速分析，以确认加工食品中食物过敏原标签的适当性。本研究旨在开发一种快速可靠的方法，同时检测痕量的七种食品致敏性蛋白（小麦，荞麦,牛奶，鸡蛋,甲壳类动物,花生,和核桃）在加工食品中使用LC-MS/MS。使用悬浮捕集（S-Trap）柱和在线自动固相萃取来改善以前使用LC-MS/MS进行过敏原分析所需的复杂且耗时的预处理过程。所开发的方法能够同时检测来自五种类型的经痕量变应原性蛋白质修正的事件样品中的七种食品成分的特定蛋白质的选定标记肽。估计每种蛋白质的方法的检测值的极限
